Naslov
Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis

Autori
Massari, Dražen ; Prpić-Massari, Larisa ; Kehler, Tatjana ; Kaštelan, Marija ; Peršić, Viktor ; Ružić, Alen ; Laškarin, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstacts / Polić Bojan - Rijeka : Hrvatsko imunološko društvo, 2011, 46-46

Skup
2011 Annual meeting of the Croatian Immunological Society

Mjesto i datum
Rabac, Hrvatska, 07.10.2011. - 09.10.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
cytotoxicity ; granulysin ; NK cell ; T cell ; psoriatic arthritis

Sažetak
Introduction: Psoriasis is generally perceived as an inflammatory cell mediated autoimmune disease, which in approximately 40% of patients progresses in psoriatic arthritis (PsA). Activated T lymphocytes are the most common inflammatory cells found in the skin and joints of PsA patients. The decreased percentage of NKT cells in the peripheral blood of psoriatic patients and their detection in plaques suggest their tissue recruitment and involvement in the pathogenesis of local manifestations of the disease. Furthermore, NK cells have also been identified in psoriatic plaques along with smaller numbers of cytotoxic NK cells expressing the activating CD16 receptor in the peripheral blood of these patients. However, the total percentage of NK cells in the peripheral blood of patients with severe disease forms is higher than in mild psoriasis. Considering the inherent and dominant cytotoxic properties of NK cells, a hypothesis has emerged regarding the involvement of cytotoxic reactions mediated by tissue-infiltrating lymphocytes in the pathogenesis of PsA. The aim of the study was to investigate possible changes in granulysin (GNLY)-mediated cytotoxicity of peripheral blood lymphocytes in psoriatic arthritis (PsA) patients with respect to different phases of the disease. Materials and methods: We prospectively enrolled 26 PsA patients in the active phase, 26 PsA patients in remission, and 26 healthy controls. The simultaneous detection of intracellular (GNLY) and cell surface antigens (CD3 and CD56) was performed with flow cytometry. Granulysin apoptotic protein was visualised by immunocytochemistry. Natural killer (NK) cell cytotoxicity was analysed with a cytotoxicity assay against human erythroleukaemia K-562 cells. Results: The percentage of GNLY+ cells in PsA patients was higher than in healthy examinees due to the increased percentage of GNLY+ cells within T cells, NKT cells, and both, and in the CD56+dim and CD56+bright NK subsets. It did not differ significantly between PsA patients in the acute phase and those in remission. However, the mean fluorescence intensity for GNLY was always higher in all lymphocyte subpopulations in the acute phase than in remission and in healthy controls. Accordingly, GNLY-mediated NK cell cytotoxicity against K-562 cells of active phase PsA patients was significantly higher than in patients in remission or in healthy controls. Conclusion: These finding demonstrated for the first time the involvement of GNLY in the worsening of PsA and suggested that GNLY could mediate the development of joint lesions.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA