Naslov
Cytotoxic Effects of Imidazolium Ionic Liquids on Fish and Human Cell Lines

Autori
Cvjetko, Marina ; Radošević, Kristina ; Tomica, Andrej ; Slivac, Igor ; Vorkapić-Furač, Jasna ; Gaurina Srček, Višnja

Izvornik
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU (0004-1254) 63 (2012), 1; 15-19

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CCO cells; HeLa cells; imidazolium ionic liquids; MTT assay

Sažetak
Ionic liquids bring a promise of a wide range of "green" applications that could replace conventional volatile solvents. However, before these applications become large-scale, their toxicity needs to be investigated in order to predict the impact on human health and environment. In this study we assessed the cytotoxicity of imidazolium ionic liquids (in the concentrations between 0.1 mmol L-1 and 10 mmol L-1) in the ovarian fish cell line CCO and the human tumour cell line HeLa using the MTT cell viability assay. Our results showed that the most cytotoxic ionic liquid was 1-n-butyl-3-methylimidazolium bis(trifluoromethylsulphonyl)imide, [BMIM][Tf2N], followed by 1-n-butyl-3-methylimidazolium tetrafluoroborate [BMIM][BF4], 1-n-butyl-3-methylimidazolium hexafluorophosphate [BMIM][PF6], and 1, 3-dimethylimidazolium hexafluorophosphate [MMIM][PF6]. Generally, the effects were concentration-dependent. They also depended on the type of anion and the n-alkyl chain length. The comparison between the fish CCO and human HeLa cell lines suggests that CCO cells provide a good biological system for initial toxicity testing of ionic liquids that could replace in vivo bioassays.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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