Pregled bibliografske jedinice broj: 536106
Intra-vacuolar replication of F. novicida within A. castellanii-pathogenesis of tularemia on in vivo model
Intra-vacuolar replication of F. novicida within A. castellanii-pathogenesis of tularemia on in vivo model // Medical Biodefense Conference 2011
München, Njemačka, 2011. (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 536106 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Intra-vacuolar replication of F. novicida within A. castellanii-pathogenesis of tularemia on in vivo model
Autori
Brezovec, Martin ; Ozanič, Mateja ; Šantić, Marina
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
Medical Biodefense Conference 2011
Mjesto i datum
München, Njemačka, 25.10.2011. - 28.10.2011
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Tularemia; Amoeba; Pathogenesis
Sažetak
Francisella tularensis is a gram negative facultative intracellular bacterium that causes zoonotic disease tularemia. Free living amoeba are environmental host of several intracellular pathogens. It has been shown that F. tularensis utilize A. castellanii as a natural reservoir. For the first time we show that in contrast to mammalian cells, where bacteria replicate in the cytosol, F. novicida resides and replicates within membrane-bound vacuoles within the trophozoites of Acanthamoeba castellanii. In contrast to the transient residence of F. tularensis within acidic compartment within A. castellanii. Based on these studies we investigated the effect of inhaled amoeba on the pathogenesis of tularemia on in vivo model. Balb/c mice were infected intratracheally with F. novicida (106 bacteria per mouse) or were coinculated with F.novicida (106 per mouse) and A. castellanii (106 amoeba per mouse). Suprisingly, coinculation with A. castellanii did not enhanced intracellular replication of F. novicida in mice. Balb/c mice coinfected with A. castellanii develop only mild pneumonia in comparison to mice infected with F. novicida alone, which resulted in severe bronchopneumonia. These studies demonstrating that amoeba play an important reservoir of F. novicida in nature but have a minor role in pathogenesis ot tularemia.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
062-0621273-0950 - Francisella tularensis-unutarstanični život i patogeneza tularemije u miša (Šantić, Marina, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka