Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro

Baraba, Anja; Želježić, Davor; Kopjar, Nevenka; Mladinić, Marin; Anić, Ivica; Miletić, Ivana

doi:10.1111/j.1365-2591.2011.01869.x

Pregled bibliografske jedinice broj: 535475

Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro

Baraba, Anja; Želježić, Davor; Kopjar, Nevenka; Mladinić, Marin; Anić, Ivica; Miletić, Ivana

Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro // International endodontic journal, 44 (2011), 7; 652-661 doi:10.1111/j.1365-2591.2011.01869.x (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 535475 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro

Autori
Baraba, Anja ; Želježić, Davor ; Kopjar, Nevenka ; Mladinić, Marin ; Anić, Ivica ; Miletić, Ivana

Izvornik
International endodontic journal (0143-2885) 44 (2011), 7; 652-661

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
comet assay; cytoxicity; Epiphany; genotoxicity; RealSeal

Sažetak
Aim To evaluate the in vitro genotoxicity and cytotoxicity of two resin-based root canal sealers and to determine the type of cell death they induce. Methodology The sealers tested were Epiphany and RealSeal. Each component of the material (Epiphany Primer, Epiphany Thinning Resin, Epiphany Sealant, RealSeal Primer, RealSeal Thinning Resin and RealSeal Root Canal Sealant), components in permutual combinations and all components mixed together were tested on human peripheral blood leucocytes using ethidium bromide/acridine orange viability staining and comet assay. Simultaneously, untreated negative control cultures were analysed in the same manner. DNA damage was evaluated following 4 h of+ treatment and after 24 h in the absence of the components of the materials. Results After 4 h of treatment, except thinning resin, each individual component and the different combinations of components induced a significant increase in DNA migration ability (P < 0.05). After 24 h, combination of primer, thinning resin and sealant of both materials caused cell death inducing intense apoptosis. After 24 h, cells exposed to Epiphany Sealant and RealSeal Root Canal Sealant, both in polymerized and unpolymerized form, exhibited a level of DNA damage that was similar to the control. Conclusions Primer and thinning resin of both resin-based root canal sealers and their combinations were cytotoxic and induced apoptosis. Both sealants had no significant effect on the viability of the human leucocytes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Dentalna medicina

POVEZANOST RADA

Projekti:
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Kašuba, Vilena, MZOS ) ( CroRIS)
065-0650444-0418 - Eksperimentalna i klinička endodontologija (Anić, Ivica, MZOS ) ( CroRIS)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Stomatološki fakultet, Zagreb

Profili:

Nevenka Kopjar (autor)