Naslov
Biodegradation of nicotine for detoxification of tobacco waste

Autori
Kopčić, Nina ; Sipos, Ildiko ; Ćosić, Ivana ; Vuković, Marija ; Briški, Felicita

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
3rd International Symposium on Environmental Management-Towards Sustainable Technologies ; Conference Proceedings / Koprivanac, Natalija ; Kušić, Hrvoje ; Lončarić Božić, Ana - Zagreb : Faculty of Chemical Engeneering and Technology, University of Zagreb, 2011, 262-267

ISBN
978-953-6470-56-3

Skup
3rd International Symposium on Environmental Management

Mjesto i datum
Zagreb, Hrvatska, 26.10.2011. - 28.10.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
nicotine; biodegradation; tobacco waste; Pseudomonas aeruginosa; Chryseomonas luteola

Sažetak
Tobacco industries produce large quantities of solid and liquid wastes. These wastes pose an important environmental problem, as some main components are harmful and toxic. They contain a high concentration of nicotine and total organic carbon. Furthermore, tobacco solid waste cannot be disposed in a landfill under European Union Regulation because nicotine is a toxic and harmful compound. The aim of this work was to identify the nicotine degrading microorganisms after successful nicotine removal by composting process. The experiment was carried out in a closed reactor (24 dm3) under adiabatic conditions at an airflow rate of 0.4 dm3 min-1 kg-1 of volatile solids during 19 days. The detoxification process during the composting was monitored by observing the nicotine concentration and changes in the total number of microorganisms of the reactor samples. Nicotine concentration was measured using high performance liquid chromatography (HPLC). Nicotine degrading bacteria were isolated from agar plates and identified using API 20 NE set. After 19 days, the raw compost was almost free of nicotine. From the raw compost were isolated and identified two bacterial species Pseudomonas aeruginosa and Chryseomonas luteola. To test that isolated bacteria are the true nicotine degraders another set of experiments were carried out using the obacco leachate. The leachate was prepared by ixing tobacco waste and tap water and filtered for batch experiments. Isolated bacteria exhibited considerably strong pollution elimination abilities, with 100% nicotine degradation and between 75 and 80% chemical oxygen demand (COD) removal within 28 h and 75 h, depending on initial nicotine concentration and the COD concentration in tobacco leachate.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

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