Naslov
Expression of pannexin familly of gap junction proteins in invasive ductal breast carcinoma

Autori
Paić, Frane ; Ramić, Snježana ; Perić Balja, Melita ; Ivčević, Sanja ; Legčević, Zoran ; Hiršl, Lea ; Bulić-Jakuš, Floriana ; Knežević, Fabijan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final program and abstracts / - Zagreb : International Society for Applied Biological Sciences (ISABS), 2011, 197-197

Skup
7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine

Mjesto i datum
Bol, Hrvatska, 20.06.2011. - 24.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
invasive ductal breast carcinoma ; pannexin 1 ; pannexin 2 ; pannexin 3

Sažetak
Breast cancer (BC) is the most prevailing malignancy among worldwide female population, with constantly increasing incidence and serious consequences on morbidity and mortality of affected patients. Genotypically and phenotypically BCs represent very heterogeneous group of tumours, diverse in their pathohistological features, expression of molecular markers, malignant/metastatic potential, clinical presentation, prognosis, and response to therapy. The purpose of this preliminary study was to determine the expression pattern of pannexin family (Panx 1/2/3) of gap junction proteins in invasive ductal breast carcinoma. A total of 27 female patients with diagnosed invasive ductal BC were included in the study. All cases were graded according to the modified criteria of Scarff Bloom-Richardson, as described by Elston and Ellis. Tumour tissue samples surgically removed from each patient (primary tumor and positive axillary lymph nodes) were embedded in paraffin blocks for immunohistological staining or snap- frozen in liquid N2 for RNA isolation. Immunohistochemical staining was performed with rabbit polyclonal anti-human pannexin-1, 2 and 3 antibody (AbCam ab78380, ab55917, ab98093) using DAB substrate and HRP reaction. RNA isolation, in vitro transcription and qRT-PCR were performed using TRI Reagent and RNeasy Mini kit, gene specific Quanti Tect Primer Assay (Qiagen) and 7500 qRT-PCR system (Applied Biosystem). Obtained results showed positive (protein, mRNA) expression pattern ranging from diffuse (Panx2) to granular (Panx1, Panx3) cytoplasmatic staining with slight or no membrane positivity in tumor cells of all analyzed samples. However, the significance, if any, of their expression for tumorigenesis of invasive ductal breast carcinoma has still to be elucidated.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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