Naslov
Ex vivo development of the rat limb bud with 5-azacytidine

Autori
Mužić, Vedrana ; Bulić-Jakuš, Floriana, Jurić-Lekić, Gordana ; Himelreich, Marta ; Majić, Željka ; Sinčić, Nino ; Katušić Bojanac, Ana ; Vlahović, Maja ; Šerman, Ljiljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Program and Abstracts of 7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine / - Zagreb : International Society for Applied Biological Sciences (ISABS), 2011, 195-195

Skup
7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine

Mjesto i datum
Bol, Hrvatska, 20.06.2011. - 24.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
5-azacytidine ; limb bud ; ex vivo ; development ; embryotoxicity

Sažetak
The aim of this investigation was to explore the developmental potential of rat limb buds in a serum-supplemented organ culture system under the influence of the DNA demethylating agent 5-azacytidine. Fisher rat fore- and hind-limb buds were removed under the dissecting microscope from 13 and 14-days-old embryos and placed on a lens paper supported by a stainless steel grid where they spent three days or two weeks at the air-liquid interface. Eagle's Mininmum Essential Medium was supplemented with 50% rat serum and used alone or with 5 􀈝m 5-azacytidine. Longer and shorter limb bud axes were measured by an ocular micrometer. Samples were processed by routine histology, embedded in parafin and uninterrupted serial sections were stained by HE, Masson trichrome or Azan stain. In isolated limb buds immature epithelium, mesenchyme, myotubes and different stages of angiogenesis such as single hemangioblasts, hemangioblast agreggates, blood islands and capillaries filled with erythroblasts were present. During the 3-day culture period, stratified epithelium and cartilage differentiated but differentiated striated muscle was not observed. In limb buds that spent two-weeks in culture, necrosis and absence of angiogenesis together with keratinization of the stratified epithelium was discovered. Limb buds treated with 5-azacytidine were smaller than controls. It can be concluded that developmental parameters such as overall growth and differentiation of specific tissues in this mammalian model system make it adequate to screen for embryotoxic substances such as the epigenetic drug 5- azacytidine used in this investigation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA