Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4-dichloroisocoumarin

Leščić Ašler, Ivana; Pigac, Jasenka; Vujaklija, Dušica; Luić, Marija; Štefanić, Zoran

doi:10.1107/S1744309111032222

Pregled bibliografske jedinice broj: 533173

Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin

Leščić Ašler, Ivana; Pigac, Jasenka; Vujaklija, Dušica; Luić, Marija; Štefanić, Zoran

Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin // Acta crystallographica. Section F, Structural biology and crystallization communications, 67 (2011), 11; 1378-1381 doi:10.1107/S1744309111032222 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 533173 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin

Autori
Leščić Ašler, Ivana ; Pigac, Jasenka ; Vujaklija, Dušica ; Luić, Marija ; Štefanić, Zoran

Izvornik
Acta crystallographica. Section F, Structural biology and crystallization communications (1744-3091) 67 (2011), 11; 1378-1381

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
streptomyces rimosus ; SGNH hydrolases ; lipases ; 3 ; 4-dichloroisocoumarin

Sažetak
A recombinant lipase (triacylglycerol acylhydrolase ; EC 3.1.1.3) from the bacterium Streptomyces rimosus was inhibited by the serine protease inhibitor 3, 4-dichloroisocoumarin and crystallized by the hanging-drop vapour-diffusion method at 291 K. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 38.1, b = 78.7, c = 56.6 Å, [beta] = 104.5° and probably two molecules in the asymmetric unit. Diffraction data were collected to 1.7 Å resolution using synchrotron radiation on the XRD beamline of the Elettra synchrotron, Trieste, Italy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
F logocrystallization communications share

POVEZANOST RADA

Projekti:
MZOS-098-0982913-2877 - Temeljna molekularno-biološka istraživanja streptomiceta (Vujaklija, Dušica, MZOS ) ( CroRIS)
MZOS-098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Luić, Marija, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Marija Luić (autor)