Naslov
Quantitative nanomechanical mapping of marine diatom in seawater by Peak Force Tapping

Autori
Pletikapić, Galja ; Berquand, Alequandre ; Mišić Radić, Tea ; Svetličić, Vesna.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
IV AFM Biomed Conference Book of Abstracts / Scheuring, Simon ; Parot, Pierre ; Pellequer, Jean-Luc - Pariz, 2011, 42-42

Skup
IV International Meeting on AFM in Life Sciences and Medicine

Mjesto i datum
Pariz, Francuska, 23.08.2011. - 27.08.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
atomic force microscopy (AFM); Cylindrotheca closterium; deformation; diatom cell wall; elasticity; marine diatom; nanomechanical properties; Peak Force Tapping AFM; Young’s modulus

Sažetak
It is generally accepted that a diatom cell wall is characterized by a siliceous skeleton covered by an organic envelope essentially composed of polysaccharides and proteins. Understanding of how the organic component is associated with the silica structure provides an important insight into the biomineralization process and patterning on the cellular level. We have selected weakly silicified marine diatom Cylindrotheca closterium. The unique characteristics of Cylindrotheca spp. cell wall are regions which are believed to be completely unsilicified. The nanomechanical properties were measured over the entire cell surface in seawater at a resolution that was not achieved in previous studies of marine diatoms. Based on nanomechanical mapping of diatom cell wall we discovered silica nanoparticles in the valve region that has been reported to be unsilicified and purely organic. A cell wall model is proposed with individual silica nanoparticles incorporated in an organic matrix. Such organization of girdle band and valve regions enables the high flexibility needed for movement and adaptation to different environments while maintaining the integrity of the cell.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija, Biotehnologija

POVEZANOST RADA