Naslov
Malassezia pachydermatis in isolates from bears and wolves

Autori
Hađina, Suzana ; Pinter, Ljiljana ; Mojčec, Vesna ; Reljić, Slaven ; Vujaklija, Duška

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Fungi and Mycotoxins in Health and Disease : Programme and abstracts / Antolović, Roberto ; Miličević, Tomislav - Zagreb : Hrvatsko mikrobiološko društvo, 2011, 64-54

ISBN
978-953-7778-01-9

Skup
Power of Fungi and Mycotoxins in Health and Disease

Mjesto i datum
Primošten, Hrvatska, 19.10.2011. - 22.10.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Malassezia pachydermatis; bears; wolves;

Sažetak
The genus Malassezia can be found on the skin and mucous of different animal species. At present dogs and cats represent the best explored group of hosts of this lipophilic yeast. On the contrary, the wild animals as carriers of Malassezia species are much less examined. This study is undertaken to isolate and characterize Malassezia species from wild animals applying traditional microbiological and molecular methods. The swabs were taken from the left and right external ear canal, and anus of the brown bears and wolves and cultured on modified Dixon agar. Grown colonies of Malassezia pachydermatis were identified based on the microscopic morphology, and subcultured on Sabouraud dextrose agar in order to confirm its non lipid dependency. Thirteen sequences from M. pachydermatis isolates were analyzed on the basis of the first internal transcribed spacer (ITS-1). DNA was isolated with Invitrogen PureLinkTM Genomic DNA mini kit. PCR products amplified with specific primers 18 SF1 (5'-AGGTTTCCGTAGGTGAACCT-3') and 5.8 SR1 (5'-TTCGCTGCGTTCTTCATCGA-3') corresponded to a 282 region that of ITS-1 sequence. Sequence analysis showed molecular homogeneity, and all isolates analyzed so far, were identified as M. pachydermatis.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA