Naslov
Detection of toxicity of aflatoxin M1 and F-2 toxin by lethal action of Artemia salina larvae

Autori
Duraković, Lejla ; Markov, Ksenija ; Čvek, Domagoj ; Frece, Jadranka ; Delaš, Frane

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Fungi and Mycotoxins in Health and Disease / Antolović, R., Miličević, T. - Zagreb : Hrvatsko mikrobiološko društvo, 2011, 55-55

ISBN
978-953-7778-01-9

Skup
3rd International Interdisciplinary Scientific Symposium, Power of Fungi and Mycotoxins in Health and Disease

Mjesto i datum
Primošten, Hrvatska, 19.10.2011. - 22.10.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Toxigenic moulds; Aflatoxin M1; F-2 toxin; Bioassay; Artemia salina

Sažetak
The chemical determination of aflatoxins and other mycotoxins in foods and feeds should be confirmed by bioassay. Many nontoxic compounds in nature fluorescence and behave chromatographically in a similar manner to the mycotoxins. A biological test to verify the chemical analysis is relatively rapid and can be conducted in 24 hours. The present biological tests for determining toxicity of mycotoxins are extremely involved lengthy procedures. In this work, temperature-dose relationship with aflatoxin M1 and F-2 toxin (zearalenone) were studied using the brine shrimp Artemia salina larvae as biological indicator. Whole pasteurized milk from retail shop was used. The milk showed no detectable levels of aflatoxin M1 (AFM1) and F-2 toxin and the toxins were added in concentration that varied from 0.05 to 10 μg/mL of milk. Artemia salina larvae were used to assess the acute toxicity of investigated mycotoxins in respect to incubation temperature and toxin concentration. Mortality was determined by counting the immobile (dead) larvae under stereoscopic microscope. The data represented are from the tests conducted at 30 oC. AFM1 at dose levels of 1.0 μg/L and above produced greater than 90% mortality after 24 hours. Purified F-2 toxin showed an order of toxicity approximately 3 times less than AFM1. Because the tests can be conducted in a volume of only 1.0 mL, a minimum amount of AFM1 and F-2 toxin can be used ; positive results, with mortality of over 50% can be achieved with 0.5 μg/L of AFM1 and 4.0 μg/L of F-2 toxin, respectively. Artemia salina larvae are especially attractive for use in a short-term bioassay for selected mycotoxins. The dry eggs can withstand adverse environmental conditions and still hatch out in 24-48 hours in the presence of artificial seawater. Continuous maintenance of stock cultures is not necessary. A continuous system of hatching can be used to provide fresh larvae daily.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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