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Pregled bibliografske jedinice broj: 530997

Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study

Roelants, Mieke; Huygens, Ann; Crnolatac, Ivo; Van Cleynenbreugel, Ben; Lerut, Evelyne; Van Poppel, Hendrik; de Witte, Peter A.M.
Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study // BJU international, 109 (2012), 2; 300-305 doi:10.1111/j.1464-410X.2011.10465.x (međunarodna recenzija, članak, znanstveni)

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Naslov
Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study

Autori
Roelants, Mieke ; Huygens, Ann ; Crnolatac, Ivo ; Van Cleynenbreugel, Ben ; Lerut, Evelyne ; Van Poppel, Hendrik ; de Witte, Peter A.M.

Izvornik
BJU international (1464-4096) 109 (2012), 2; 300-305

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Evans blue; non-muscle-invasive bladder cancer; transmission electron microscopy; white-light cystoscopy; bladder cancer diagnosis; spheroids

Sažetak
We found that Evans blue preferentially accumulate in spheroids prepared from urothelial cell carcinoma (UCC) cells as compared to spheroids composed of normal human urothelial (NHU) cells. The present findings could be important for future developments in clinical diagnostics for early bladder cancer detection staging and grading involving white light cystocopy. OBJECTIVE • To develop a diagnostic method relying on the preferential accumulation of a dye in non-muscle-invasive bladder cancer (NMIBC) that is visible in conjunction with white-light cystoscopy (WLC).MATERIALS AND METHODS • We investigated in detail the permeation of Evans blue in urothelial cell carcinoma (UCC) spheroids prepared from T24, J82 and RT-112 human cell lines and spheroids composed of normal human urothelial (NHU) cells.• To gain more insight into the differential accumulation, all spheroids were investigated ultrastructurally using transmission electron microscopy (TEM).RESULTS • We found that, after exposure to Evans blue for 2 h, UCC spheroids accumulated dramatically more dye than spheroids composed of NHU cells.• Using TEM it was found that the malignant spheroids contain similar ultrastructural characteristics, i.e. a wide intercellular space and a decreased number of desmosome-like cell attachments, to those from clinical samples of non-papillary carcinoma in situ of the bladder.CONCLUSION • We believe the present findings could be important for future developments in clinical diagnostics for early bladder cancer detection, staging and grading involving WLC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Ivo Crnolatac (autor)

Poveznice na cjeloviti tekst rada:

doi onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Roelants, Mieke; Huygens, Ann; Crnolatac, Ivo; Van Cleynenbreugel, Ben; Lerut, Evelyne; Van Poppel, Hendrik; de Witte, Peter A.M.
Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study // BJU international, 109 (2012), 2; 300-305 doi:10.1111/j.1464-410X.2011.10465.x (međunarodna recenzija, članak, znanstveni)
Roelants, M., Huygens, A., Crnolatac, I., Van Cleynenbreugel, B., Lerut, E., Van Poppel, H. & de Witte, P. (2012) Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study. BJU international, 109 (2), 300-305 doi:10.1111/j.1464-410X.2011.10465.x.
@article{article, author = {Roelants, Mieke and Huygens, Ann and Crnolatac, Ivo and Van Cleynenbreugel, Ben and Lerut, Evelyne and Van Poppel, Hendrik and de Witte, Peter A.M.}, year = {2012}, pages = {300-305}, DOI = {10.1111/j.1464-410X.2011.10465.x}, keywords = {Evans blue, non-muscle-invasive bladder cancer, transmission electron microscopy, white-light cystoscopy, bladder cancer diagnosis, spheroids}, journal = {BJU international}, doi = {10.1111/j.1464-410X.2011.10465.x}, volume = {109}, number = {2}, issn = {1464-4096}, title = {Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study}, keyword = {Evans blue, non-muscle-invasive bladder cancer, transmission electron microscopy, white-light cystoscopy, bladder cancer diagnosis, spheroids} }
@article{article, author = {Roelants, Mieke and Huygens, Ann and Crnolatac, Ivo and Van Cleynenbreugel, Ben and Lerut, Evelyne and Van Poppel, Hendrik and de Witte, Peter A.M.}, year = {2012}, pages = {300-305}, DOI = {10.1111/j.1464-410X.2011.10465.x}, keywords = {Evans blue, non-muscle-invasive bladder cancer, transmission electron microscopy, white-light cystoscopy, bladder cancer diagnosis, spheroids}, journal = {BJU international}, doi = {10.1111/j.1464-410X.2011.10465.x}, volume = {109}, number = {2}, issn = {1464-4096}, title = {Evans blue as a selective dye marker for white-light diagnosis of non-muscle-invasive bladder cancer : an in vitro study}, keyword = {Evans blue, non-muscle-invasive bladder cancer, transmission electron microscopy, white-light cystoscopy, bladder cancer diagnosis, spheroids} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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