Naslov
Safety and immunogenicity of HVT vaccine against Marek's disease applied in hatchery by nebulization

Autori
Mazija, Hrvoje ; Čajavec, Stanislav ; Nemarnik, Djurdja ; Prukner-Radovčić, Estella ; Ragland, William, L.

Izvornik
Poultry Science Annual meeting Abstracts. 89th Annual Meeting August 18-20, Montreal Canada

Vrsta, podvrsta
Ostale vrste radova, ostalo

Godina
2000

Ključne riječi
Marek's disease; herpesvirus of turkey; nebulization; safety test; immunogenicity

Sažetak
Marek's disease (MD) is controled by vaccines containing specific lentogenic strains or related herpesvirus of turkey (HVT). All vaccines nowdays are applied parenterally. The in ovo inoculation techniques offers another possibility. The purpous of the study was to evaluate safety and immunogenicity of HVT vaccine applied by ultrasound nebulization to newly hatched chickens.//Aerosol Device SONOVAC 095 creates aerosol of HVT water-suspension, 95% of particles range 2 to 5 microns in diameter. One hundred chickens at once are exposed to aerosol for 30 seconds. Vaccine used had a titer od 2000 pfu per dose and was diluted in a distilled water. Groups of chickens, A (25) and B (49) were exposed to aerosol of HVT 1 or 15 minutes, and K (23) was given distilled water. In a second trial the vaccine was given to 4, 400 chickens by nebulization and 3, 800 birds subcutaneously. Vaccinated chickens remaind in a hatchery for 24 hours and, during 20 weeks, were placed in two separate parts in the same poultry house. Viremia test was performed at day 10. There wos no clinical reaction to the given HVT in chickens exposed to aerosol for 1 or 15 minutes. Vaccinated layers didn't shaw any negative vaccinal reaction. Viremia was proved positive at day 10, and precipitins where present in 100% of blood sera. The used HVT vaccine was safe and immunogenic in both experiments. The small particle of aerosol did not cause any negative vaccinal reaction. Compared to parenteral vaccination, mnortality rate was much lower (3.7% and 6.06%), probably because there was no stress reaction caused by injection.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA