Pregled bibliografske jedinice broj: 529197
Intracellular replication of F. novicida within H. vermiformis
Intracellular replication of F. novicida within H. vermiformis // 16th International Congress of the Hungarian Society for Microbiology : abstracts
Budimpešta, Mađarska, 2011. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 529197 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Intracellular replication of F. novicida within H. vermiformis
Autori
Ožanič, Mateja ; Semić, Vildana ; Pavoković, Gordana ; Mrvčić, Valentina ; Abu Kwaik, Yousef ; Šantić, Marina
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
16th International Congress of the Hungarian Society for Microbiology : abstracts
/ - , 2011
Skup
International Congress of the Hungarian Society for Microbiology (16 ; 2011)
Mjesto i datum
Budimpešta, Mađarska, 20.07.2011. - 22.07.2011
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
Tularemia; amoeba; phagosome
Sažetak
Francisella tularensis is a gram negative facultative intracellular bacterium that causes the zoonotic disease tularemia. Free-living amebae, such as Acanthamoeba and Hartmannella, are environmental hosts of several intracellular pathogens. Epidemiology of F. tularensis in various parts of the world is associated with water-borne transmission, which includes mosquitoes and amebae as the potential host reservoirs of the bacteria in water resources. In vitro studies showed intracellular replication of F. tularensis within A. castellanii cells. Whether ameba is a biological reservoir for Francisella in the environment is not known. We used Hartmannella vermiformis as an amebal model system to study the intracellular life of F. novicida. For the first time we show that F. novicida survives and replicates within H. vermiformis. The iglC mutant strain of F. novicida is defective for survival and replication not only within A. castellanii but also in H. vermiformis cells. In contrast to mammalian cells, where bacteria replicate in the cytosol, F. novicida resides and replicates within membrane-bound vacuoles within the trophozoites of H. vermiformis. In contrast to the transient residence of F. novicida within acidic vacuoles prior to escaping to the cytosol of mammalian cells, F. novicida does not reside transiently or permanently in an acidic compartment within H. vermiformis when examined 30 min after initiation of the infection. We conclude that F. tularensis does not replicate within acidified vacuoles and does not escape into the cytosol of H. vermiformis. The Francisella pathogenicity island locus iglC is essential for intra-vacuolar proliferation of F. novicida within H. vermiformis. Our data show a distinct intracellular lifestyle for F. novicida within H. vermiformis compared to mammalian cells.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
062-0621273-0950 - Francisella tularensis-unutarstanični život i patogeneza tularemije u miša (Šantić, Marina, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Marina Šantić
(autor)
Mateja Ožanič
(autor)
Gordana Pavoković
(autor)
Valentina Marečić
(autor)