Naslov
Morphological characteristics of chicken lymphocytes vaccinated against Marek's disease using HVT FC 126 by means of nebulization and parenteral vaccination

Autori
Kardum, Matko ; Poljičak-Milas, Nina ; Gottstein, Željko ; Milinković-Tur, Suzana ; Ciglar Grozdanić, Irena ; Mazija, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
BAC Journal of the British Association for Cytopathology, 22 (Suppl.1), pp. 1-194. Abstracts of the 36th European Congress of Cytology / Herbert, A. - Oxford : Willey-Blackwell, 2011, 138-138

Skup
36th European Congress of Cytology

Mjesto i datum
Istanbul, Turska, 22.09.2011. - 25.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Marek's disease; immunocompetent cells’s transformation; image analysis

Sažetak
Marek's disease is one of the major problems in poultry production today and specific vaccination is one of the most important modes of its prevention. The aim of this study was to compare morphological patterns of chicken's lymphocytes in peripheral blood, before and on the 3rd, 4th, 7th, 10th, 14th and 21st day after vaccination by means of either nebulization or parenteral injection. Image analysis (area, convex area, outline, maximum and minimum radius, length and breadth, as well as form factor and elongation factor) was performed using the SFORM software (VAMSTEC, Zagreb). A total of 50 blood smears of vaccinated chickens (20 by means of nebulization and 30 by parenteral injection), with an average of 100 cells per smear, were analysed. The results showed that peripheral blood lymphocytes of chickens vaccinated by means of nebulization compared with parenteraly vaccinated were significantly larger in most examinated variables: area, outline, minimal and maximal radius, convex area, length and breadth, form factor as well as elongation factor of the whole cell (regarding lymphocyte cytoplasm) and nuclei, on the 3rd, 4th, 7th, 10th, 14th and 21st day after vaccination. In lymphocytes of parenteraly vaccinated chickens, only the nucleo-cytoplasmic ratio was higher. The results show morphological patterns of immunocompetent cell transformation, which can be used to evaluate immune responses to vaccination and vaccine efficacy. We conclude that nebulization as a mode of vaccination against Marek’s disease stimulates the transformation of immunocompetent cells much earlier, thus shortening the time of immunosupresion and increasing the immune response.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA