Naslov
Complex structural composition of accumulated GD1a species in brain tissue of GD3 synthase knockout mice

Autori
Marinčić, Dragana ; Mlinac, Kristina ; Heffer, Marija ; Zamfir, Alina ; Vukelić, Željka ; Kalanj Bognar, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SiNAPSA Neuroscience Conference (SNC'11) : Central European FENS Featured Regional Meeting : Book of Abstracts / Osredkar, Damjan ; Koritnik, Blaž ; Pelko, Miha - Ljubljana : Slovenian Neuroscience Association (SiNAPSA), 2011, 140-140

ISBN
978-961-91704-4-1

Skup
SiNAPSA Neuroscience Conference

Mjesto i datum
Ljubljana, Slovenija, 22.09.2011. - 25.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
brain gangliosides; mass spectrometry; GD3

Sažetak
Gangliosides, glycosphingolipids containing sialic acids, are found with great diversity and variety in mammalian central nervous system. Many of the physiological functions of gangliosides have been elucidated using knock-out (KO) mouse models. The aim of the study was to systematically characterize the structures of brain gangliosides in St8sia1 KO mice in comparison to wild-type (wt) mice. The St8sia1 mice lack GD3 synthase with consequent deficiency in b-series gangliosides. In this study, frontocerebral tissue of 3 St8sia1 and 3 wt mice was homogenized and used for total ganglioside extraction. Separation of ganglioside mixture was performed using high-performance thin-layer chromatography (HPTLC), also utilized for preparative isolation of separated fractions for subsequent mass spectrometry (MS) analysis. MS screening and sequencing analyses (MS1 ; MS/MS) were performed on a High Capacity Ion Trap Ultra mass spectrometer coupled with a NanoMate robot incorporating ESI 400 Chip technology. All mass spectra were acquired in the m/z range 100-3000. Biochemical phenotype of St8sia1 mice was confirmed by finding complete absence of GD3, GD2, GD1b, GT1b and accumulation of GD1a and GM1. In accumulated GD1a HPTLC migrating fraction from St8sia1 mice brain, a higher number and proportion of individual molecular species comparing to the wt was determined by MS. Detected GD1a species differed by a composition of the ceramide moiety and a higher abundance of acetylated and fucosylated monosialo/disialo-ganglioside structures was revealed. The presence of described ganglioside structures in brain tissue of St8sia1 mice indicates structural modifications i.e. possible alternative synthetic pathways as a consequence of GD3 synthase deficiency.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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