Naslov
Comparison of ractopamine and clenbuterol accumulation in pigs as meat production animals

Autori
Pleadin, Jelka ; Vulić, Ana ; Perši, Nina ; Vahčić, Nada ; Milić, Dinka

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the 7th International Congress of Food Technologists, biotechnologists and nutritionists / Medić, Helga - Zagreb : Hrvatsko društvo prehrambenih tehnologa, biotehnologa i nutricionista, 2011, 317-321

ISBN
978-953-99725-4-5

Skup
7th International Congress of Food Technologists, biotechnologists and nutritionists

Mjesto i datum
Opatija, Hrvatska, 20.09.2011. - 23.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ractopamine; clenbuterol; accumulation; tissues of pigs; meat production

Sažetak
Ractopamine and clenbuterol are β-adrenergic agonists that increase the rate of weight gain and feed conversion. If used in meat production, they promote muscle growth of treated animals by increasing nitrogen retention, protein synthesis and lipolysis, and suppressing lipogenesis. They can be illegally used during fattening of farm animals, thus implying a risk to consumers of meat and meat products. For residue surveillance of both substances, it is essential to choose appropriate matrices and use high sensitivity analytical methods in the analysis of suspect tissues. The aim of the study was to compare residue levels and accumulation potential of ractopamine and clenbuterol in pig tissues, liver and kidney (n=24), after subchronic repeat administration of clenbuterol and 10-fold dose of ractopamine. Ractopamine and clenbuterol residues in both matrices were determined using validated enzyme-linked immunosorbent assay (ELISA) as a quantitative screening method. Method validation showed good mean recoveries (R >70%) with acceptable inter- and intra-day relative standard deviations (RSD <10%) for both analytes and matrices. Immediately after the last dose of β-agonists, the maximal determined ractopamine concentration in the kidney (42.44 ng/g) was 2-fold that measured in the liver (20.66 ng/g) and was comparable with maximal concentration of clenbuterol in the liver (47.43 ng/g). Considering the dose difference, study results pointed to higher accumulation of clenbuterol residues in both matrices, suggesting the liver to have a significantly higher accumulation potential for clenbuterol residues and kidney for ractopamine residues. The results also suggested these tissues to be appropriate matrices to detect ractopamine and clenbuterol abuse in meat production.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA