Naslov
Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Autori
Pavoković, Dubravko ; Poljuha, Danijela ; Horvatić, Anita ; Ljubešić, Nikola ; Hagège, Daniel ; Krsnik-Rasol, Marijana

Izvornik
PLANT CELL TISSUE AND ORGAN CULTURE (0167-6857) 108 (2012), 1; 111-119

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Beta vulgaris; cellular ultrastructure; cell wall; electrophoresis; extracellular proteins; plastids

Sažetak
Normal (N), habituated nonorganogenic (HNO), and tumour (T) sugar beet cell lines were analysed in order to establish specific patterns of extracellular proteins and iden- tify protein markers that might explain the distinct pheno- typical characteristics. Electron microscopy showed that the ultrastructure of N cells corresponds to that of parenchyma cells, and that these cells contain plastids with large starch grains. HNO and T cells had enlarged, lobed nuclei with an increased number of nucleoli ; the number of nuclei in HNO cells was greater than in T cells. The T plastids were elon- gated, with reduced thylakoids and abundant phytoferritin deposits, while HNO plastids were small and vacuolated, with an irregular, underdeveloped thylakoid system. The extra- cellular proteome of the cells was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Greater differences in protein expression were observed between the HNO and N lines than between the T and N lines. Sixteen of the most prominent bands differentially expressed among the cell lines were cut out from the gel and analyzed by mass spectrometry. Cell wall-modifying enzymes were identified, including a peroxidase whose expression was twofold higher in N and T tissue than in HNO tissue ; pectinesterase, which was expressed at a level threefold lower in the T line than in the other cell lines ; and xyloglucan endotransglucosylase, which was expressed at a level sixfold higher in HNO and T tissue. Three proteins belonged to the chitinase gene family and their expression was higher in HNO and T tissue than in N tissue. The differential expression of these proteins suggests that these play a role in cell line-specific cell wall composition and cell-to-cell adhesion.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA