Naslov
Neuroimmunomodulative Properties of Dipeptidyl Peptidase IV/CD26 in a TNBS-induced Model of Colitis in Mice

Autori
Batičić, Lara ; Detel, Dijana ; Kučić, Natalia ; Buljević, Sunčica ; Pernjak Pugel, Ester ; Varljen, Jadranka

Izvornik
Journal of cellular biochemistry (0730-2312) 112 (2011), 11; 3322-3333

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
dipeptidyl peptidase IV/CD26; CD26 deficient mice; TNBS-induced colitis; neuropeptide Y; vasoactive intestinal peptide; iInterleukin-6; interleukin-10

Sažetak
Causal connections between dipeptidyl peptidase IV (DPP IV/CD26) and inflammatory bowel disease (IBD) have been shown, but mechanisms of these interactions are unclear. Our hypothesis was that DPP IV/CD26 could affect the neuroimmune response during inflammatory events. Therefore, we aimed to evaluate its possible role and the relevance of the gut-brain axis in a model of IBD in mice. Trinitrobenzenesulfonic acid-induced (TNBS) colitis was induced in CD26 deficient (CD26-/-) and wild type (C57BL/6) mice. Pathohistological and histomorphometrical measurements were done. Concentrations and protein expressions of DPP IV/CD26 substrates neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) were determined. Concentrations of IL-6 and IL-10 were evaluated. Investigations were conducted at systemic and local levels. Acute inflammation induced increased serum NPY concentrations in both mice strains, more enhanced in CD26-/- mice. Increased NPY concentrations were found in colon and brain of C57BL/6 mice, while in CD26-/- animals only in colon. VIP and IL-6 serum and tissue concentrations were increased in both mice strains in acute inflammation, more pronouncedly in CD26-/- mice. IL-10 concentrations, after a decrease in serum of both mice strains, increased promptly in CD26-/- mice. Decreased IL-10 concentration was found in brain of C57BL/6 mice, while it was increased in colon of CD26-/- mice in acute inflammation. DPP IV/CD26 deficiency affects the neuroimmune response at systemic and local levels during colitis development and resolution in mice. Inflammatory changes in the colon reflected on investigated parameters in the brain, suggesting an important role of the gut-brain axis in IBD pathogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA