Naslov
Exposure to environmental tobacco smoke in interaction with ADAM33 polymorophisms increases the risk of asthma in Croatian schoolchildren

Autori
Kljaić Bukvić, Blaženka ; Blekić, Mario ; Aberle, Neda ; Ferić, Monika ; Simpson, Angela ; Custovic, Adnan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Annual Meeting of the Austrian Society for Allergology and Immunology in co-operation with the national societies from Croatia, Czech Republic, Hungary, Slovakia and Slovenia

Mjesto i datum
Beč, Austrija, 03.12.2010. - 05.12.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ADAM33 ; asthma ; tobacco smoke exposure

Sažetak
Background: A disintegrin and metalloproteinase 33 (ADAM33) was the first positional cloning asthma gene, which is associated with astma and bronchial hyperresponsivenes. Significant associations have previously been reported between single nucleotide polymorphisms (SNPs) in ADAM33 and asthma in ethnically diverse populations. Exposure to environmental tobacco smoke may contribute to developement of asthma .The aim of study was to investigate the association between ADAM33 SNPs and asthma amongst Croatian schoolchildren and interactions with environmental tobacco smoke (ETS) exposure. Methods: 424 children with asthma aged 6 to 18 years (cases), were recruited into the study from the local hospital if the following criteria were met: (1) physician-diagnosed asthma, (2) asthma symptoms within the previous 12 months, and (3) use of antiasthma medication ; 415 non- asthmatic controls were randomly selected from non- asthmatic children attending the outpatient department or local schools. ETS exposure was ascertained by validated questionnaire. We genotyped 32 haplotype tagging SNPs in ADAM33 (Sequenom). Results: All SNPs were in Hardy- Weinberg equilibrium (p>0.01). We found a significant association between nine SNPs and asthma (p<0.03). For two of these SNPs (rs487377 and rs 2485700), there was a significant interaction between genotype and ETS exposure. In the multivariate analysis for rs2485700 (adjusted for gender), we found a statistically significant interaction between the genotype and ETS exposure (p=0.03), in that the risk of asthma was increased amongst carriers of the minor allele, but only amongst smokers (aOR 2.1, 95% CI 1.4- 3.2, p<0.001), with no increase in risk amongst non- smokers (0.9, 0.5-1.8, p=0.9). Similarly, in the multivariate analysis for rs487377 (adjusted for gender), we found a statistically significant interaction between the genotype and ETS exposure (p=0.04), with the risk of asthma increasing amongst the minor allele homozygotes amongst smokers (3.7, 1.5-9.4, p=0.006), but not amongst non-smokers (0.8, 0.2-2.6, p=0.7). Conclusions: Variants in ADAM33 were associated with asthma in Croatian schoolchildren. In addition, we have identified significant interaction between genotype and ETS exposure, with the risk of asthma increasing in specific genotypes only amongst children exposed to ETS.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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