Naslov
Interaction of silver nanoparticles with cholinesterases

Autori
Šinko, Goran ; Vinković Vrček, Ivana ; Miljanić, Snežana ; Dijanošić, Adriana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 36th FEBS Congress, Biochemistry for Tomorrow's Medicine ; in: The FEBS Journal 278 (2011) (S1) / - : Wiley-Blackwell, 2011

Skup
36th FEBS Congress, Biochemistry for Tomorrow's Medicine

Mjesto i datum
Torino, Italija, 25.06.2011. - 30.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
silver nanoparticles; acetylcholinesterase; butyrylcholinesterase; inhibition

Sažetak
The environmental impact of nanomaterials is expected to increase substantially in the near future as they are becoming a part of our daily life. Silver nanoparticles (AgNP) are widely used in the form of cosmetics, food packaging, drug delivery systems, therapeutics, antimicrobial agents, biosensors, or labels. Manufactured nanoparticles can be toxic via interactions with proteins and enzymes. Nanoparticles are known to be able to interfere with cellular metabolism and to cause cytotoxicity and moreover may interfere with specific cellular functions. We studied interaction of AgNP with acetylcholinesterase (AChE) and related butyrylcholinesterase (BChE). Both cholinesterases are present in brain, blood and nervous system. Acetylcholinesterase (AChE) is a key enzyme in cholinergic neurotransmission and inhibition of AChE may lead to fatal outcome. BChE has important pharmacological and toxicological roles due to its hydrolysis of various xenobiotics. We have evaluated the ChE inhibition by AgNPs prepared using reduction methods of silver nitrate with trisodium citrate (AgNP-citrate), hydroxylamine hydrochloride (AgNP-chloride) and sodium borohydride in presence of polyvinyl alcohol (AgNP-PVA). All tested AgNPs inhibited ChEs and the evaluated apparent inhibitor constants ranged from 49 μM to 670 μM. In the case of AChE the inhibition potency increased in the following order: AgNP-PVA < AgNP-citrate < AgNP-chloride, while in the case of BChE the inhibition potency increased in the following order: AgNP-citrate < AgNP-PVA < AgNP-chloride. The impact of AgNP on ChE activity depends greatly on the nature of the nanomaterial coatings. It is also interesting that AgNP showed higher affinity for AChE, than for BChE.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA