Naslov
Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input

Autori
Udiković-Kolić, Nikolina ; Devers-Lamrani, Marion ; Petrić, Ines ; Hršak, Dubravka ; Martin-Laurent, Fabrice

Izvornik
Applied microbiology and biotechnology (0175-7598) 90 (2011), 4; 1547-1554

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
atrazine; degradation; bacterial culture; evolution; atz genes; trz genes

Sažetak
We evaluated the effects of variations in atrazine input on the evolution of a bacterial culture adapted to a low atrazine concentration. This initial culture (M3-K) was subjected to weekly subculturing in the presence of a high concentration of atrazine as the only N source (100 mg l−1). After four subculturing, M3-K evolved to a new bacterial culture (M3) which exhibited a significant increase in the extent of atrazine mineralization in comparison with the initial culture. Molecular analyses of M3-K and M3 cultures by cloning, restriction analysis, and sequencing of the 16S rRNA genes revealed significant differences in culture structure and composition. M3-K culture comprised mainly Actinobacteria (40%), β-Proteobacteria (26%), and Bacteroidetes (16%). After exposure to a high atrazine concentration, the dominance of Actinobacteria decreased (14%), Bacteroidetes increased (27%), and β-Proteobacteria were replaced by γ-Proteobacteria (32%). Quantitative PCR revealed that the abundance of atzB and atzC genes relative to total bacteria decreased by a factor of 3–4 following the increase in atrazine concentration, while the relative abundance of trzD increased significantly (≈400 times). Presented study shows that variations in atrazine input drive both functional and compositional shifts in the atrazine-degrading bacterial culture.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA