Naslov
Nontuberculous mycobacteria and unspecific tuberculin skin reaction in cattle in Croatia

Autori
Špičić, Silvio ; Katalinić-Janković, Vera ; Zdelar-Tuk, Maja ; Duvnjak, Sanja ; Račić, Ivana ; Habrun, Boris ; Kompes, Gordan ; Vujnović, Anja ; Cvetnić, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book, ESM, Annual Congress of the European Society of Mycobacteriology / PD Dr. Stefan Niemann - Borstel : Agentur KONSENS GmbH, 2011, 80-81

ISBN
978-3-00-035094-8

Skup
Annual Congress of the European Society of Mycobacteriology

Mjesto i datum
Lübeck, Njemačka, 26.06.2011. - 29.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Nontuberculous Mycobacteria; cattle; Croatia

Sažetak
In 2010, Croatia initiated the proceedure of verification of officially tuberculosis- free herds. Cattle older than 6 weeks were tested with tuberculin skin test (monotest) twice in period of at least 6 months between testings. Cattle with suspicious reaction on monotest were re-tested using comparative method (bovine and avian tuberculin). All skin tests were performed according to the European Council Directive 64/432/EEC and Croatian ordinance on animal health requirements applicable to trade in bovine animals and swine from year 2008. Cattle with positive tuberculin skin test reactions (monotest or comparative method) were removed from breeding. Tissue and organ samples were taken during slaughter for bacteriological isolation of causative agents of bovine tuberculosis– Mycobacterium (M.) sp. Isolates were identified by microscopic examination of suspicious colonies, as well as molecular examintion for the presence of 16SrRNA. GenoType MTBC Kit (Hain, Germany) was used for identification of species belonging to the M. tuberculosis complex. Additional identification of nontuberculous mycobacteria (NTM) isolates was performed by classical bacteriological methods. Total of 314 cattle samples from from 100 breedings were examined. M. tuberculosis complex species (M. bovis, M. caprae and M. tuberculosis) were isolated from 117 cattle samples (37.3%) coming from 23 breedings (23%) in 7 counties. NTM were isolated from samples collected from 12 cattle (3.8%) belonging to 12 flocks (12%) and were: M. terrae (4), M. gordonae (1), M. peregrinum (1), M. chitae (1), M. nonchromogenicum (1), M. parafortuitum (1), M. vaccae (1), M. thermoresistibile (1) and one unindentified species (M. sp.). Mycobacteria were not isolated in 185 (58.9%) bovine samples taken from animals with positive tuberculin reaction. Bacterial infection with any kind of mycobacteria was not identified in 64 breedings (64%). Breedings in which identified causative agent of bovine tuberculosis belonged to the M. tuberculosis complex were free of NTM. Infection of cattle with numerous NTM could partially be the reason for lowered tuberculin skin test specificity and should be concidered in planing of programms like verification of cattle herds officially free from tuberculosis.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

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