Naslov
Antioxidant capacity of medicinal plants: a comparative study using five in vitro antioxidant assays

Autori
Šamec, Dunja ; Kremer, Dario ; Piljac, Ante ; Valek Žulj, Lidija ; Martinez, Sanja ; Piljac Žegarac, Jasenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
YISAC 2011- 18th Young Investigators Seminar on Analytical Chemistry- Book of Abstracts / Guzsvany, Valeria ; Papp, Zsigmond - Novi Sad : Faculty of Sciences, University of Novi Sad, 2011, 75-75

Skup
YISAC 2011- 18th Young Investigators Seminar on Analytical Chemistry

Mjesto i datum
Novi Sad, Srbija, 28.06.2011. - 01.07.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant; medicinal plant

Sažetak
In addition to playing an important role in the growth, development and protection of plant organisms, natural antioxidants may account for the recognized health benefits of various plants and plant-based foods. The overall antioxidant potency of plant extracts is influenced by many factors, such as their reducing capacity, free radical scavenging potential, metal chelating ability and the ratio of lipophyllic-hydrophillic antioxidants present in the sample. It is difficult to develop a single measure that will account for multiple mechanisms of action of natural antioxidants, therefore, it is necessary to perform more than one type of antioxidant assay when attempting to deduce the antioxidant potency of a sample. In the present study we measured the antioxidant capacity of Jiaogulan (Gynostemma pentaphyllum) – a traditional Chinese remedy for diabetics and two Croatian endemic plants used in traditional medicine to treat mange, skin diseases, inflammation and as purgatives and for blood detoxication - Micromeria croatica (Pers.) Schott. and Rhamnus intermedia Steud. et Hochst, respectively. We compared the results obtained from five different antioxidant tests: three spectrophotometric assays (Ferric Reducing/Antioxidant Power assay (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, and 2, 2´-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay), as well as cyclic voltammetry and oxygen radical absorbance capacity (ORAC). The antioxidant potency composite index was calculated (ACI) for all tested medicinal plants as a way of incorporating the absolute values from all four antioxidant capacity assays into one measure. We will discuss the differences between the results obtained from different tests and between different plant species. Correlations will be drawn between various assay results.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA