Naslov
Evaluation of steroid hormones anabolic use in cattle in Croatia

Autori
Pleadin, Jelka ; Terzić, Svjetlana ; Nina, Perši ; Vulić, Ana

Izvornik
Biotechnology in animal husbandry (1450-9156) 27 (2011), 2; 147-159

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
steroid sex hormones ; anabolics ; physiological levels ; plasma ; bovine
(steroid hormones ; anabolics ; physiological levels ; plasma ; bovine)

Sažetak
The hormone concentration exceeding the physiological ones, found on monitoring fot illegal substance use, would point to the abuse of these substances for anabolic purpose. In the present study, concentration of the natural hormones 17 beta-estradiol, progesterone and testosterone were determined in bovine plasma according to animal sex and age. Natural hormone concentration were determined using quantitative validated ELISA methods in plasma samples from cattleof different breed composition collected at several farms in Croatia during the 2006-2009 period. Method validation showed good mean recovery and repeatibility (approx. 75-87%), demonstrating the methods efficacy in determination of 17 beta-estradiol, progesterone and testosterone level in cattle plasma, respectively. The level of sex hormones was statistically significantly higher in yearling plasma as compared with plasma as compared with calf plasma (P<0.05). The highest levels of 17 beta-estradiol (0.03+/-0.01 ng/ml)and progesterone (4.87+/-5.47 ng/ml) in male yearlings. Results showed the steroid hormone levels to vary with animal age and sex, indicated that illegal use of anabolic substances could not be suspected in none of study animals.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija

POVEZANOST RADA