аЯрЁБс>ўџ 68ўџџџ5џџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџьЅС#`№ПЫbjbjmЅmЅ.ЯЯО џџџџџџЄЈЈЈЈЈЈЈМ€€€€” МЇЖЌЌЌЌЌЌЌЌ$]hХ44-ЈTЌЌTT4ЈЈЌЌaВВВTЈЌЈЌВTВВЈЈВЌ  ј'3ЗУЫ€j(Вw0ЇВљ’љВљЈВ\ЌРВЮ кzЌЌЌ44Ј ЌЌЌЇTTTTМММФ€МММ€МММЈЈЈЈЈЈџџџџ Influence of stress, antibiotics and NSAID therapy on health, welfare and semen quality in Holstein-Frisian bulls Nikica Prvanovi Babi, B. Radiai, Marija Lipar, Ivanka Maji Bali, A. Orak, M. Samard~ija, J. Grizelj, D. Mati i, P. Bo~i, M. Cergolj Holstein-Frisian bulls are predominant in semen production in dairy industry. They are regularly kept and managed with maximal care and in optimal envronment. Unfortunately, their size and body frame increases due to selection. Increse of body mass and size of the animals makes boxes and stanchions too small and could lead to stress and environment-linked deseases. In this study it was monitored group of 11 Holstein-Frisian bulls kept in boxes and stanchions of Unit for semen production, which was originally built for Simmental bulls. Although boxes still meet prefessional standards, some of the largest bulls(n=5) developed ulcera and blisters on claws and distal extremities due to inadequate living space. On the beginning of the trial these animals were treated by surgical removal of ulcera, claw correction and supportive therapy (antibiotics and NSAIDs as painkillers, if needed). The other bulls (control group, n=6) only had claw correction. All 11 bulls were monitored daily for next 90 days following surgery. Blood samples were collected weekly. Hemogram and biochemical profile of the animal was proceded and analysed immidiatly. Sera samples were also stored on-20АC and used later to determine levels of testosterone and cortisol. After short interval of recovery, bulls were used for semen collection and spermiological and andrological tests were performed after each ejaculation. Recovery interval depended on healing process and varied from 72 h (claw corection only) till 30 days (surgical removal of blisters and ulcera followed with supportive therapy). In 2 bulls surgery was repeated after 70 days since ulcera reoccured. According to clinical and laboratory findings only repeated surgery bulls needed and received NSAID therapy to remove pain. They recieved flunixin-meglumine 2.5 mg/kg IV / 24 h during 3 consequtive days.According to laboratory data bulls after surgery and during supportive therapy (n=5) had altered hemogram and biochemical parameters specific for reversibile renal demage and endotoxaemia (altered total proteins, creatinin, urea, lymphopenia) for more than 30 days although clinically appeared healthy. There wasn't noted significant diference (p<0,01) between treated (n=5) and control group (n=6) neither for cortisol nor in testosterone level. Also, it wasn't noticed any significant change neither in spermiogram nor in andrological test results. There wasn't noticeble any difference for bulls (n=2) that were reoperated and received strong antibiotic and NSAID therapy for 3-5 days. тф  $ & ( b d ј њ ќ ( L р 3 l t ‚ ˜ И а ё ѓ  f j Щ Ъ ш э њ | } М Н ъ я ясгЩМЩМЩМЩМЩВЊЂš’š’Š‚’z’Š’z’z‚zr‚j‚rj‚r‚rhЅ_ŠB*phhЋ§B*phhз,–B*phhЗDЫB*phhV B*phhчB*phhѓMŒB*phhЅнB*phhйT–B*phhйT–6B*phhr-^hr-^6B*phhr-^6B*phhr-^hr-^6>*B*phh+Gч5B*CJaJph hr-^hr-^5B*CJaJph)фњ ќ ЩЪЫїїїяїї$a$gdЗDЫ$a$gdr-^Ыўя [hq|}ПР.\suЈЋИ№ё@6Qopƒбйъэ/7JMiv|ГѓШЫїяїяїчпчпїчїзЯчзчзЯЧЯзчпчпчпчпчпчпчпчпчпПНЧЕhѓMŒB*phUhV B*phhrхB*phh›pB*phhьŒB*phhќi B*phhЅ_ŠB*phhЋ§B*phhЗDЫB*ph,Still, as mentioned above, all bulls that were cured for ulcera and blisters had mild endotoxemia although appeared healthy, returned back to semen collection and gave ejaculat of acceptable quality. ,1hА‚. АЦA!А‰"А‰#‰$‰%ААФАФ Ф†œ@@ёџ@ NormalCJ_HaJmHsHtH>AђџЁ> Zadani font odlomkaViѓџГV Obi na tablicaі4ж l4жaі .kєџС. 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