Naslov
Accumulation of ß-agonists clenbuterol and salbutamol in black and white mouse hair

Autori
Vulić, Ana ; Pleadin, Jelka ; Perši, Nina ; Stojković, Ranko ; Ivanković, Siniša

Izvornik
Journal of analytical toxicology (0146-4760) 35 (2011), 8; 566-570

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ß-agonists; mice; black/white hair; ELISA

Sažetak
Hair has been shown to be an excellent site for the accumulation of different drugs including ß-agonists, and therefore it would be an appropriate matrix for surveillance for the presence of drug residues. The aim of this study was to determine concentrations and to compare accumulation of two different ß-agonists in black and white mice hair by use of ELISA as a screening quantitative method. The study included 200 8-week-old white and black mice. One group of black mice and one group of white mice were treated with clenbuterol in a dose of 2.5 mg/kg body mass per os for 28 days. Other animals were treated in the same way with salbutamol. The highest (±SD) clenbuterol concentration of 631.4±23.5 ng/g in black hair and 228.5±156.2 ng/g in white hair was determined on day 1 of treatment withdrawal. Study results revealed the black to white hair ratio of clenbuterol accumulation to be 1:2-1:4 and of salbutamol accumulation 1:1.4. The mean (±SD) salbutamol concentrations determined on day 1 of treatment withdrawal was 23.9±0.9 ng/g and 16.4±1.1 ng/g in black and white hair samples, respectively. The study demonstrated that residues could be determined in hair samples even after 30 days of withdrawal period.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Biotehnologija

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