Naslov
Indeterminate Results of QuantiFERON-TB Gold In-Tube Assay in Nonimmunosuppressed Children

Autori
Zrinski Topić, Renata ; Zoričić-Letoja, Ivka ; Pavić, Ivan ; Dodig, Slavica

Izvornik
Archives of medical research (0188-4409) 42 (2011), 2; 138-143

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
children; indeterminate result; interferon-gamma; phytohemagglutinin; tuberculosis infection

Sažetak
QuantiFERON-TB Gold In Tube (QFT-IT) assay is a highly sensitive and specific test for the diagnosis of latent tuberculosis infection. Data on the use of QFT-IT assay in children are scarce and contradictory. The aim of the study was to assess the rate of indeterminate test results and to identify the factors contributing to indeterminate results on routine use of QFT-IT assay in non-immunosuppressed children. This retrospective study included 2173 children, age range from 1 month to 18 years. Determination of interferon-gamma (IFN-γ) in peripheral blood was performed by commercial QFT-IT assay. Indeterminate test results were recorded in ten (0.46%) subjects with ages ranging from 15 months to 15 years. The value of negative control was >8.0 kIU/L in one subject, whereas in the remaining nine subjects indeterminate results were consequential to positive control (<0.50 kIU/L). None of these subjects had any history data on congenital or acquired immunodeficiency disorders. Bacterial infection (with elevated body temperature and therapy with β-lactam antibiotics) was present in eight subjects with indeterminate results, one subject had exacerbation of asthma (therapy with inhalation corticosteroids), and one subject was clinically healthy. Repeat IFN-γ determination, performed in seven subjects, did not yield indeterminate results. Study results showed the rate of indeterminate QFT-IT results in non-immunosuppressed children of all age groups to be very low. QFT-IT should preferably be performed upon resolution of acute inflammation in order to avoid repeat testing of indeterminate results in a new blood sample and to reduce the cost of testing.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA