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Pregled bibliografske jedinice broj: 512642

Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92

Beganović, Jasna; Frece, Jadranka; Kos, Blaženka; Leboš Pavunc, Andreja; Habjanič, Ksenija; Šušković, Jagoda
Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92 // Antonie van leeuwenhoek international journal of general and molecular microbiology, 100 (2011), 1; 43-53 doi:10.1007/s10482-011-9563-4 (međunarodna recenzija, članak, znanstveni)

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Naslov
Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92

Autori
Beganović, Jasna ; Frece, Jadranka ; Kos, Blaženka ; Leboš Pavunc, Andreja ; Habjanič, Ksenija ; Šušković, Jagoda

Izvornik
Antonie van leeuwenhoek international journal of general and molecular microbiology (0003-6072) 100 (2011), 1; 43-53

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lactobacillus helveticus M92; Probiotic; S-layer protein; Salmonella

Sažetak
The objective of this study was the characterisation of the S-layer protein (SlpA) and its functional role in the probiotic activity of Lactobacillus helveticus M92. SlpA was isolated and identified by SDS-PAGE LC-MS/MS analysis. The slpA gene encoding the SlpA from L. helveticus M92 was sequenced and compared with other well characterised slpA genes. Sequence similarity searches revealed high homology with the SlpA of Lactobacillus strains. Purified SlpA showed significantly better immunomodulatory effects in orally immunised mice than L. helveticus M92 cells after SlpA removal. SlpA is involved in the autoaggregation of L. helveticus M92 cells and coaggregation of L. helveticus M92 with S. Typhimurium FP1 as these processes were negatively affected after SlpA removal from the cell surface. Therefore, the influence of oral treatment with L. helveticus M92 on an oral infection of mice by S. Typhimurium FP1 was investigated. Following the oral immunization of mice, with viable L. helveticus M92 and S. Typhimurium FP1 cells, the concentration in the luminal contents of total S-IgA and specific anti-Salmonella S-IgA antibodies, from all immunized mice was significantly higher compared to the control group or a group of mice infected only with S. Typhimurium FP1. These results demonstrate that the observed reduced infection by S. Typhimurium FP1 in mice with L. helveticus M92 is associated with competitive exclusion in the intestinal tract and enhanced immune protection conferred by the L. helveticus M92 and its SlpA.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA

Projekti:
058-0581990-2007 - Probiotici, prebiotici i funkcionalne starter kulture (Šušković, Jagoda, MZOS ) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Jadranka Frece (autor)

Avatar Url Jasna Novak (autor)

Avatar Url Blaženka Kos (autor)

Avatar Url Ksenija Uroić (autor)

Avatar Url Jagoda Šušković (autor)

Avatar Url Andreja Leboš-Pavunc (autor)

Poveznice na cjeloviti tekst rada:

doi www.springerlink.com

Citiraj ovu publikaciju:

Beganović, Jasna; Frece, Jadranka; Kos, Blaženka; Leboš Pavunc, Andreja; Habjanič, Ksenija; Šušković, Jagoda
Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92 // Antonie van leeuwenhoek international journal of general and molecular microbiology, 100 (2011), 1; 43-53 doi:10.1007/s10482-011-9563-4 (međunarodna recenzija, članak, znanstveni)
Beganović, J., Frece, J., Kos, B., Leboš Pavunc, A., Habjanič, K. & Šušković, J. (2011) Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92. Antonie van leeuwenhoek international journal of general and molecular microbiology, 100 (1), 43-53 doi:10.1007/s10482-011-9563-4.
@article{article, author = {Beganovi\'{c}, Jasna and Frece, Jadranka and Kos, Bla\v{z}enka and Lebo\v{s} Pavunc, Andreja and Habjani\v{c}, Ksenija and \v{S}u\v{s}kovi\'{c}, Jagoda}, year = {2011}, pages = {43-53}, DOI = {10.1007/s10482-011-9563-4}, keywords = {Lactobacillus helveticus M92, Probiotic, S-layer protein, Salmonella}, journal = {Antonie van leeuwenhoek international journal of general and molecular microbiology}, doi = {10.1007/s10482-011-9563-4}, volume = {100}, number = {1}, issn = {0003-6072}, title = {Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92}, keyword = {Lactobacillus helveticus M92, Probiotic, S-layer protein, Salmonella} }
@article{article, author = {Beganovi\'{c}, Jasna and Frece, Jadranka and Kos, Bla\v{z}enka and Lebo\v{s} Pavunc, Andreja and Habjani\v{c}, Ksenija and \v{S}u\v{s}kovi\'{c}, Jagoda}, year = {2011}, pages = {43-53}, DOI = {10.1007/s10482-011-9563-4}, keywords = {Lactobacillus helveticus M92, Probiotic, S-layer protein, Salmonella}, journal = {Antonie van leeuwenhoek international journal of general and molecular microbiology}, doi = {10.1007/s10482-011-9563-4}, volume = {100}, number = {1}, issn = {0003-6072}, title = {Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92}, keyword = {Lactobacillus helveticus M92, Probiotic, S-layer protein, Salmonella} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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