Pregled bibliografske jedinice broj: 51216
A Continuous Spectrophotometric Assay for Measuring Amino Acid Activation by Peptide Synthetases
A Continuous Spectrophotometric Assay for Measuring Amino Acid Activation by Peptide Synthetases // Periodicum biologorum, 101 (1999), 349-354 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 51216 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A Continuous Spectrophotometric Assay for Measuring Amino Acid Activation by Peptide Synthetases
Autori
Pavela-Vrančič, Maja ; Dieckmann, Ralf ; von Doehren, Hans
Izvornik
Periodicum biologorum (0031-5362) 101
(1999);
349-354
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Sažetak
Background and purpose: Peptide synthetases are multifunctional enzyme complexes catalysing the non-ribosomal biosynthesis of a variety of bioactive peptide products. The activation reaction is generally monitored using the isotope exchange assay, which suffers several disadvantages such as discontinuous monitoring and the use of radioactive reagents. A continuous spectrophotometric assay was introduced for the measurement of PPi generation accompanying the amino acid activation reaction catalysed by peptide synthetases.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
177050
Ustanove:
Prirodoslovno-matematički fakultet, Split
Profili:
Maja Pavela-Vrančić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
