Naslov
The survival of different Klebsiella pneumoniae strains in A549 cells

Autori
Štimac, Igor ; Štifanić, Andrea ; Vasiljev Marchesi Vanja ; Tomljenović Morana ; Rukavina, Tomislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Liječnički vjesnik / Anić, Branimir - Zagreb : Medicinska naklada, 2010, 45-45

Skup
10th ZIMS Zagreb International Medical Summit

Mjesto i datum
Zagreb, Hrvatska, 11.11.2010. - 14.11.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Klebsiella pneumoniae strains; A549 cells; Lipopolysaccaride

Sažetak
Background: The airway epithelium is the largest surface of the respiratory tract and it is often initial site of contact between microbes and their hosts. The capacity of the epithelial cells to detect the respiratory microbial pathogens and directly participate in the defence against them remains poorly investigeted. To investigate the role of lipopolysaccharide (O antigen) in the interaction between the alveolar epithelial cells and bacteria, we used Klebsiella pneumoniae (K. pneumoniae), a major nosocomial pathogen causing pneumonia. Materials and methods: In this experiments were used nine different acapsular referent K. pneumoniae strains with different O antigens (O1, O2ac, O3, O4, O5, O7, O8, O9, O12), and K. pneumoniae strain Caroli (O1:K2) containing capsular antigen K2. The A549 cells (5x105) were infected with 5x107 of different bacterial strains (MOI 1:100). Throughout the experiment were observed the adherence and the internalisation after 2, 24 and 48 hours. In the selected time points, the infected cells were sacrified and the number of viable bacteria was determined by plating the serial dilutins on blood agar plates. The experiments were done in three independent repeats. Results: Highly virulent strain K. pneumoniae Caroli does not adhere nor menage to be internalised by A549 cells. In contrast to virulent strain, acapsular strains were able efficiently adhere to lung epithelial cells. With the exeption of O8 K. pneumonia antigen strain, other acapsular strains were efficintly internalised by A549 cells. O4, O1, O7 and O2ac K. pneumoniae strains were able to multply in the lung epithelial cells, while O5, O9, O3 were degraded by A549 cell during 24 or 48 hours. Conclusions: This study shows that different O K. pneumoniae antigens diferently interact with lung epithelial cells. Unlike acapsular Klebsiella strains, K. pneumoniae Caroli does not interact with A549 cells which is probably due to the large capsular polysaccharide that surrounds the bacteria.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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