Pregled bibliografske jedinice broj: 509060
Sevoflurane and isoflurane abolished the phrenic long term facilitation in rats
Sevoflurane and isoflurane abolished the phrenic long term facilitation in rats // European Journal of Anaesthesiology
Amsterdam, Nizozemska, 2011. str. 73-73 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 509060 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Sevoflurane and isoflurane abolished the phrenic long term facilitation in rats
Autori
Marinov, Vjera ; Karanović, Nenad ; Pavlinac, Ivana ; Valić, Maja ; Pecotić, Renata ; Đogaš, Zoran
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
European Journal of Anaesthesiology
/ - , 2011, 73-73
Skup
Euroanaesthesia 2011, The European Anaesthesiology Congress
Mjesto i datum
Amsterdam, Nizozemska, 11.06.2011. - 14.06.2011
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
anaesthetic volatile; hypoxia; research animal
Sažetak
Phrenic long-term facilitation (pLTF) is manifested by prolonged increase in phrenic nerve activity after episodes of acute intermittent hypoxia (AIH). The goal of the study was to explore the effects of sevoflurane and isoflurane monoanaesthesia at equal MAC values on induction of pLTF in rats, compared to urethane anaesthesia. After Biomedical Ethics Committee approval, Sprague-Dawley adult, male rats, BM of 300-350 g were used. Two experimental groups of nine animals each (sevoflurane and isoflurane) and one control group of seven animals (urethane) were formed. After achieving adequate depth of anaesthesia animals were tracheotomysed, mechanically ventilated and bilaterally vagotomised. Femoral veins and arteries were cannulated for saline infusion, blood pressure monitoring and arterial blood sampling. Phrenic nerve was dissected and recorded. After obtaining the planned level of anaesthesia (1.4 MAC for sevoflurane and isoflurane and 1.2 mg/kg for urethane intraperitoneally), animals were exposed to AIH protocol (5x3 min hypoxic episodes with FiO₂=0.09, separated by 3 min pause with FiO₂=0.5). The same level of anaesthesia was maintained to the end of the experiment. Peak phrenic nerve amplitude (PNA), respiratory frequency (f), and breathing rhythm parameters were analyzed during hypoxic episodes and at 15, 30, and 60 min after the last hypoxic episode and compared to baseline values (immediately before the first hypoxic episode). Data are presented as mean±SEM. A repeated measure ANOVA was used for analysis. Statistical significance was set at P<0.05. In both experimental groups average PNA decreased at 60 min after the last hypoxic episode compared to baseline values, i.e. pLTF was abolished. In sevoflurane group PNA decreased by 31.0±11.0% (P<0.01) and in isoflurane group decreased by 44.6±12.7% (P<0.01). In control (urethane) group average PNA increased by 108.7±25.3% (P<0.01) 60 min after the last hypoxic episode compared to baseline values, i.e. pLTF was induced. During all five hypoxic episodes average PNA increased in both experimental groups and in control group compared to baseline values, i.e. hypoxic ventilatory response (HVR) was preserved. Sevoflurane and isoflurane monoanaesthesia at 1.4 MAC abolished the pLTF compared to urethane anaesthesia.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
216-2163166-0513 - Neuralna kontrola disanja u budnosti i spavanju (Đogaš, Zoran, MZOS ) ( CroRIS)
216-2163166-3342 - Središnja regulacija kardiovaskularnog i respiracijskog sustava-uloga serotonina (Valić, Maja, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Split
Profili:
Renata Pecotić
(autor)
Maja Valić
(autor)
Zoran Đogaš
(autor)
Ivana Pavlinac Dodig
(autor)
Nenad Karanović
(autor)