Naslov
Spectrophotometric Determination of N-Acetyl-L-Cysteine and N-(2-Mercaptopropionyl)-Glycine in Pharmaceutical Preparations

Autori
Kukoc-Modun, Lea ; Radić, Njegomir

Izvornik
International Journal of Analytical Chemistry (1687-8760) Vol. 2011 (2011); 1-6

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
N-acetyl-L-cysteine ; N-(2-Mercaptopropionyl)-Glycine ; spectrometric detection

Sažetak
A simple spectrophotometric method for determination of N-acetyl-L-cysteine (NAC) and N-(2-mercaptopropionyl)glycine (MPG) in pharmaceutical preparations was developed, validated and used. The proposed equilibrium method is based on a coupled two step redox and complexation reaction. In the first step, Fe(III) is reduced to Fe(II) by NAC or MPG. Subsequently, Fe(II) is complexed with 2, 4, 6-tripyridyl-s-triazine (TPTZ). Several analytical parameters of the method were optimized for NAC and MPG analysis in the concentration range from 1.0  10-6 to 1.0  10-4 mol L-1. Regression analysis of the calibration data showed a good correlation coefficient (0.9999). The detection limit of the method was 1.4  10-7 mol L-1 for NAC and 1.3  10-7 mol L-1 for MPG. The method was successfully applied to quantify NAC and MPG in pharmaceutical preparations. No interferences were observed from common pharmaceutical excipients.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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