Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

Balen, Biljana; Tkalec, Mirta; Šikić, Sandra; Tolić, Sonja; Cvjetko, Petra; Pavlica, Mirjana; Vidaković-Cifrek, Željka

doi:10.1007/s10646-011-0633-1

Pregled bibliografske jedinice broj: 505587

Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

Balen, Biljana; Tkalec, Mirta; Šikić, Sandra; Tolić, Sonja; Cvjetko, Petra; Pavlica, Mirjana; Vidaković-Cifrek, Željka

Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc // Ecotoxicology, 20 (2011), 4; 815-826 doi:10.1007/s10646-011-0633-1 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 505587 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

Autori
Balen, Biljana ; Tkalec, Mirta ; Šikić, Sandra ; Tolić, Sonja ; Cvjetko, Petra ; Pavlica, Mirjana ; Vidaković-Cifrek, Željka

Izvornik
Ecotoxicology (0963-9292) 20 (2011), 4; 815-826

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cadmium; zinc; Lemna minor L.; oxidative stress; DNA damage

Sažetak
The effects of 5 μM cadmium (Cd), a non-essential toxic element and 25 and 50 μM zinc (Zn), an essential micronutrient, were investigated in aquatic plant Lemna minor L. after 4 and 7 days of exposure to each metal alone or to their combinations. Both metals showed tendency to accumulate with time, but when present in combination, they reduced uptake of each other. Cd treatment increased the lipid peroxidation and protein oxidation indicating appearance of oxidative stress. However, Zn supplementation in either concentration reduced values of both parameters, while exposure to Zn alone resulted in elevated level of lipid peroxidation and protein oxidation but only on the 7th day. Enhanced DNA damage, which was found on the 4th day in plants treated with Cd alone or in combination with Zn, was reduced on the 7th day in combined treatments. Higher catalase activity obtained in all treated plants on the 4th day of experiment was reduced in Zn-treated plants, but remained high in plants exposed to Cd alone or in combination with Zn after 7 days. Cd exposure resulted in higher peroxidase activity, while Zn addition prominently reduced peroxidase activity in the plants subjected to Cd stress. In conclusion, Cd induced more pronounced oxidative stress and DNA damage than Zn in applied concentrations. Combined treatments showed lower values of oxidative stress parameters— lipid peroxidation, protein oxidation and peroxidase activity as well as lower DNA damage, which indicates alleviating effect of Zn on oxidative stress in Cd-treated plants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA

Projekti:
073-0731674-1673 - Utjecaj oksidativnog stresa na organizaciju i funkciju biljnih stanica i tkiva (Lepeduš, Hrvoje, MZOS ) ( CroRIS)
119-0982934-3110 - UČINAK ONEČIŠĆENJA NA GENETIČKU STRUKTURU ORGANIZAMA U VODENOM OKOLIŠU (Klobučar, Goran, MZOS ) ( CroRIS)
119-1191196-1200 - Diferencijalna ekspresija proteina u biljnim stanicama (Balen, Biljana, MZOS ) ( CroRIS)
119-1191196-1202 - Molekularni mehanizmi odgovora hrvatskih endemičnih i rijetkih biljaka na stres (Pevalek-Kozlina, Branka, MZOS ) ( CroRIS)