Naslov
Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer

Autori
Popović Hadžija, Marijana ; Kapitanović, Sanja ; Radošević, Senka ; Čačev, Tamara ; Mirt, Mirela ; Kovačević, Duje ; Lukač, Josip ; Hadžija, Mirko ; Spaventi, Radan ; Pavelić, Krešimir

Izvornik
Journal of Molecular Medicine (0946-2716) 79 (2001), 2-3; 128-132

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Colon cancer; Tumor suppressor gene; DPC4; Loss of hetrozygosity

Sažetak
We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human spordic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem repeat (VNTR) analysis and three polymorfic markers. From 36 analyzed samples 35 (97%) were heterozygous or informative. Loss of heterozygosity at the DPC4 locus was detected in 18(51%) of informative tumor DNAs. The DPC4 LOH was more frequent in smaller tumors(<5 cm) than in larger ones. Thre was no correlation between DPC4 LOH and age or sex of patients. There was a negative correlation between DPC4 LOH and histological grade or DUKES' stage of tumors, but without statistic significance, Observed results were in agreement with the view that malignant progression is concequence of many genetic changes. it can be concluded that inactivation of the DPC4 gene plays a role in a multistep process of outhrowth and progression of colon cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA