Naslov
Isolation of Active Substances from the Seeds of the Plant Milk Thistle (Silybum marianum) and Determination of Antioxidant Activity

Autori
Gašo-Sokač, Dajana ; Kovač, Spomenka ; Bušić, Valentina

Izvornik
Kemija u industriji : časopis kemičara i tehnologa Hrvatske (0022-9830) 60 (2011), 9; 441-445

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
silymarin ; silibinin ; DPPH radical scavenging ; antioxidant activity

Sažetak
Isolation of silymarin, a mixture of well-defined flavonolignans, from the seeds of milk thistle (Silybum marianum) was achieved. Silymarin were extracted from defatted seed with acetone without heating ; the yield was 4.1 %. The antioxidant activity of silymarin was determined at ambient temperature by means of a 2, 2-diphenyl-1- picrylhydrazyl (DPPH) colorimetry with a detection scheme at 515 nm. The activity was evaluated by the decrease in absorbance as the result of a DPPH radical color change from purple to yellow. The results obtained showed that ascorbic acid was a substantially more powerful antioxidant than the silymarin, but silymarin was a significantly stronger quencher of DPPH radical than the standard silibinin.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Prehrambena tehnologija

POVEZANOST RADA