Naslov
FADD Null Mouse Embryonic Fibroblasts Undergo Apoptosis after Photosensitization with the Silicon Phthalocyanine Pc 4

Autori
Nagy, Biserka ; Yeh, Wen-Chen ; Mak, Tak W. ; Chiu, Song-Mao ; Šeparović, Duška

Izvornik
Archives of Biochemistry and Biophysics (0003-9861) 385 (2001), 1; 194-202

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
apoptosis; ceramide; FADD; PDT; Pc 4; TNH-alpfa

Sažetak
Oxidative stress, such as photodynamic therapy with the silicon phthalocyanine Pc 4 (Pc 4-PDT), can induce apoptosis and tumor necrosis factor alpha (TNF) production. TNF receptors, as well as other death receptor, have been implicated in stress-induced apoptosis. To assess directly the role of FADD, a death receptor-associated protein, in induction of apoptosis post-Pc 4-PDT, embryonic fibroblasts from FADD knock out (k/o) and wild-type (wt) mice were used. Pc 4-PDT induced casp-3 activation and apoptosis in both cell types. In the presence of zVAD, a pan-caspase inhibitor, Pc 4-PDT-induced apoptosis was abrogated in both cell lines. Fumonisin B1 (FB), an inhibitor of ceramide synthase, had no effect on apoptosis after Pc 4-PDT in eitherl cell line. Simiral to Pc 4-PDT, exogenous C6-ceramide bypassed FADD deficiency and induced zVAD-sensitive and FB-insensitive. Induced ceramide levels remained elevated after cotreatment with TNF and zVAD in FADD wt cells. Taken together, these data provide genetic evidence for a lack of FADD requirement in Pc 4-PDT- or C6-ceramide-induced apoptosis. FB-sensitive ceramide production accompanies, but does not suffice, for apoptosis after Pc 4 photosensitization of TNF.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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