Naslov
Phytochemical Analysis and Antimicrobial Activity of Cardaria draba L. Desv. volatiles

Autori
Radonić, Ani ; Blažević, Ivica ; Mastelić, Josip ; Zekić, Marina ; Skočibušić, Mirjana ; Maravić, Ana

Izvornik
Chemistry & biodiversity (1612-1872) 8 (2011), 6; 1170-1181

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cardaria draba; Brassicaceae; glucosinolates; isothiocyanates; antimicrobial activity; GC-MS

Sažetak
Two different volatile isolates from aerial parts of Cardaria draba (L.) Desv, were obtained by hydrodistillation and dichloromethane extraction and analysed by gas chromatography/gas chromatography-mass spectrometry. The main volatiles obtained by hydrodistillation originated from glucoerucin degradation, i.e. 4-(methylthio)butyl isothiocyanate (28.0%) and 5-(methylthio)pentanenitrile (13.8%). Also, volatiles without sulfur and/or nitrogen were identified, mostly hexadecanoic acid (10.8%), phytol (10.2%), dibutylphatalate (4.5%) and some other compounds in smaller percentages. Volatile isolate obtained by dichloromethane extraction after exogenous myrosinase hydrolysis contained mostly compounds originating from glucosinolate degradation. They originated from glucoraphanin (4-(methylsulfinyl)butyl isothiocyanate (69.2%) and 5-(methylsulfinyl)pentanenitrile (4.5%)) glucosinalbin (4-hydroxyphenylacetonitrile (7.2%)) and glucoerysolin (4-(methylsulfonyl)butyl isothiocyanate (5.0%)). The volatile samples were evaluated for antimicrobial activity using the disc diffusion method and determining minimum inhibitory concentrations. All volatile isolates expressed a wide range of growth inhibition activity against both Gram-positive and Gram-negative bacteria and fungi. The minimum inhibitory concentrations varied between 4 and 128 µg/ml.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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