ࡱ> 5?4j7 #9bjbjUU 0V7|7|5l>$>$>$>$&&&&///80:04&oWnz0p00001]2,2OOOOiO~rS~V$X ZDW&21122WI5>$>$00)WI5I5I52>$0&0OI52OI5I5:Ft%&N0n0 gQvm&L /G3QNFN?W0oWNFA[G3A[NI5&&>$>$>$>$ Web-based virtual endoscopy Sven Loncaric and Tomislav Markovinovic Faculty of Electrical Engineering and Computing, University of Zagreb Unska 3, 10000 Zagreb, Croatia e-mail: sven.loncaric@fer.hr Abstract. A web-based application for computed tomography (CT) image analysis and visualization of the bronchial airways is presented in the paper. The technique used in this application provides a non-invasive way to examine the interior of the bronchial tubes. Many different properties of the tubes such as presence of the foreign objects or structure abnormalities of the tubes can be detected this way. This method can also be used for other similar structures, such as blood vessels. The application uses the CT images obtained by spiral computed tomography as input. Amplitude thresholding is used for segmentation of the airways. The medial axis of the airways has been used as the path for animation. The procedure produces a fly-through animation in the MPEG format, which is obtained by volume rendering of the original CT images along the extracted path. The user can choose the appropriate path using the Virtual Reality Modeling Language (VRML) model, which is sufficient for visualization of basic structures. The algorithms and the software have been developed on the SUN Ultra 1workstation. Introduction Virtual reality techniques can be applied in many areas of human activity [1]. Medicine is a very large field with many uses for virtual reality (VR) techniques, in particular for VR visualization. The reason for this is the complexity of the human body which makes visualization difficult or nearly impossible in many cases since it requires a complex three-dimensional (3-D) structure representation, and representation of spatial relationship between different structures. Virtual endoscopy (VE) is a new technique for visualization of internal structures that has some advantages over classical endoscopy (CE) [2]. In CE, the endoscope is inserted in the patient body through a natural or minimally invasive opening on the body. A disadvantage of CE is its invasiveness and a fact that only a limited number of structures can be visualized. VE is not invasive (except radiation produced by CT) and enables visualization of any structure. A disadvantage of VE is the lack of texture and color of the tissue which is a very important diagnostic information. METHODS AND PROCEDURES In this paper we present a VE technique based on CT image analysis which provides a non-invasive way for examination of the bronchial tubes. The proposed technique can be used for visualization of other tubular structures, too. The technique works in a client-server WWW environment. The user communicates with the system by means of a WWW browser which provides computer platform independence. All computation is performed on the WWW server side and results presented to the WWW client. The web-based application presented in this paper consists of several steps. In the first step, a set of chest CT images is uploaded to the server by the user. The 3-D segmentation of airways is performed in the second step. In the next step, a 3-D skeleton of the bronchial tubes is extracted from the 3-D segmented airways. Skeletons of complex 3-D objects have complex topological structure, therefore a VRML model of airway structure is used for desired axis selection. The selected axis is smoothed using the B-splines, which are very often used for 3-D animations, and used as a path for a fly-through animation of the airways. Volume rendering is then used for the visualization of the airway structure. Before making the final animation, there is a preview available. Key frames of the animation are rendered and presented to the user before computing the complete animation. SEGMENTATION Segmentation of medical images is a challenging task [3]. It is impossible to use a single segmentation method for all classes of medical images. Here, the segmented image is required only for skeleton computation and not for visualization itself. Therefore it possible to use a simple segmentation method without loosing any valuable information. Since endoscopy is used to examine airways, blood vessels or other hollow structures, they can be recognized as black or very dark areas on CT images, which makes threshold segmentation a good choice for this task. The problem is that this technique results in segmented airways but also other undesired objects. To overcome this difficulty user interaction is required, where the user chooses the desired structure.  EMBED Word.Picture.8 Figure  SEQ Figure \* ARABIC 1 An original chest CT image (left), manually segmented image (center), and amplitude threshold segmentation (right). Figure 1 shows an original chest CT image and two segmentation results. The first segmented image is a result of manual segmentation, which should be very close to real structure. The second segmented image is obtained by thresholding. The resulting skeletons computed for both images are very similar. The skeleton computation is performed in 3-D with different aspect ratios in X, Y, and Z axes. SKELETON COMPUTATION A 3-D skeleton of the bronchial tubes is extracted from the 3-D segmented structure. The skeleton is used as a path for fly-through animation of the airways or other structures. Many thinning algorithms use distance transformation as the first step [4]. A value that represents the distance from the nearest edge of the 3-D shape is assigned to every point of the distance map. Background points are assigned zero value. Since the space is discrete, approximations are necessary. Most distance transformations use a 3x3x3 scanning mask, which produces satisfactory results for many applications. Although the results are more accurate with 5x5x5 or even bigger neighborhood, the algorithms are complex and slow with almost no visible difference. In this paper an algorithm is presented that uses a 3x3x3 mask to scan the image. For each of the 27 voxels in the 3-D mask, the distance must be defined from central voxel to other voxels. There are 6 voxels with distance equal to 1, 12 voxels with distance equal to  EMBED Equation.3  and 8 voxels with distance equal to  EMBED Equation.3 . Since integer values are used in the transformation, the basic values are multiplied with 100. The mask in the form of the three two-dimensional layers is shown in Figure 3 (left). 173 141 173 141 100 141 173 141 173 141 100 141 100 0 100 141 100 141 173 141 173 141 100 141 173 141 173173 141 173 141 100 140 173 140 172 141 100 140 100 0 99 141 99 140 173 141 172 141 99 140 172 140 172Figure  SEQ Figure \* ARABIC 2. The masks for standard (left) and modified (right) distance transformation. The result of thinning in the discrete space is often not equal to actual 3-D axis that can be obtained in continuous space. In particular, the thickness of the obtained skeleton may not be equal to one. For example, a 4x4x10 solid geometric figure does not have a unique skeleton in discrete environment. If a modified definition of a mask is introduced it is possible to obtain a medial axis that has thickness equal to one. In this work we have used the definition shown in Figure 3 (right). Distances from the central voxel to the opposite voxels are not the same which artificially makes one of the voxels closer to the edge and thus the other voxel becomes a part of the medial axis. This results in a skeleton that has thickness equal to one. Each of the thirteen axes that go through the center has asymmetric value from the opposite sides of the central voxel. The difference is very small in order to prevent the phase jump, which could happen if the object is large enough. In that case larger values would be used. Except the above differences the algorithm is the same as other distance transform algorithms. After the data is transformed, a 3x3x3 neighborhood is used to compute the skeleton. In 3-D environment the skeleton is orthogonal to the 2-D plane in which the central point has the maximum value. For every voxel the algorithm checks if the voxel has the maximum value in any of the 2-D plane. If such a voxel is found it is then marked as the skeleton voxel. In most cases only the planes along the three basic axes must be checked. Including the diagonal axis produces many extra voxels, which makes it more difficult to determine the final axis along the object of interest. This is another problem of the discrete space, since in the continuous space, only the three major axis are sufficient. VRML VISUALIZATION The 3-D segmentation obtained by thresholding can be very complex with many branches. Every branch produces its skeleton, which makes it difficult to find the global axis. The bronchial tube usually has two major skeleton branches and many smaller ones. It is up to the user to choose the axis for creation of fly-through animation. Recently, VRML has become a standard way for description of 3-D models used in VR applications [5], [6]. In the proposed procedure each 3-D voxel belonging to the segmented airway structure is represented by a point in the VRML model. Skeleton points are represented by small spheres, each with its own number. The number can be seen by bringing the cursor in the VRML browser over the sphere. The user chooses the first and the last voxel of the desired skeleton. The shortest distance between these two voxels is calculated using the marked voxels as a path. The method eliminates unnecessary branches. After the first and the last voxels are chosen, it is necessary to find marked voxels between them. This is performed using the Dijkstra algorithm, which finds the shortest path between any two voxels. The results in the final animations created using this method are very good. The obtained axis is smoothed using B-splines interpolation. VOLUME RENDERING VISUALIZATION Volume rendering has become an increasingly used technique for visualization of volume data in recent years [7]. The advantage of volume rendering is in realistic visualization of volume data and ability of technique to simulate object opacity. As opposed to surface rendering no object surface detection and no triangulation is necessary in volume rendering. The disadvantage of volume rendering is in computational requirements and the lack of graphical hardware acceleration devices. Another problem is low resolution of medical images, which is often the case. Volume rendering handles the problem of low resolution data much better than surface rendering. A standard volume rendering algorithm has been used in this application, except for some modifications. The ray that travels from the user eye-point into the structure looses its power and gets weaker as it passes through semitransparent voxels. The calculation for each ray stops when its power is under some minimal value given by the user, which increases the computation speed. Most of the structures that can be viewed using this application are tube-like structures and the direction of the camera is always towards the end of the tube. This means that rays close to the edge of the rendered image travel smaller distance than the ones in the middle. The differences of rendered pixels near the edge are very small so there is no need to calculate a value for each ray. Simple interpolation can be used for edge rays, without any visible loss in quality. In the middle, rays travel far from the eye-point. When they reach their end, the distance between two neighboring rays is very large making the calculated values different. Therefore, interpolation cannot be used for pixels in the middle of the rendered image. The example of the volume rendered images is shown in Figure 3. Conclusion In this paper, an on-line web-based implementation of the algorithm used to generate interactive virtual endoscopy visualization of 3-D medical structures. The procedure performs analysis of CT medical images that the user uploads to the server. The bronchial structure of interest is segmented. A skeleton of the segmented structure is computed to serve as the animation path for fly-through animation. The virtual endoscopy application is useful both for educational and diagnostic purposes. The software is developed on a SUN Ultra 1 workstation in C++, HTML, PHP and VRML languages. Figure  SEQ Figure \* ARABIC 3. Examples of volume rendered virtual endoscopy bronchial views. REFERENCES [1] G. Burdea, Virtual Reality Technology, John Wiley, 1994 [2] R. A. Robb, Virtual (computed) endoscopy: Development and evaluation using the Visible Human datasets, Presented at the Visible Human Project Conference, October 7-8, 1996, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, URL: http://www.nlm.nih.gov/research/visible/vhp_conf/robb/robb_pap.htm [3] A. P. Dhawan and S. Juvvadi, Knowledge-Based Analysis and Understanding of Medical Images, Computer Methods and Programs in Biomedicine, 33:221-239, 1990 [4] G. Borgefors, Distance Transformations in Arbitrary Dimensions, Computer Vision, Graphics, and Image Processing, 27:321-345, 1984 [5] A. L. Ames, D. R. Nadeau, J. L. Moreland. The VRML 2.0 Sourcebook. New York: Wiley, 1996. [6] VRML 97 Specification, International Standard ISO/IEC 14772-1:1997 , The Virtual Reality Modeling Language (VRML), http://www.web3d.org/technicalinfo/specifications/vrml97/index.htm [7] M. Levoy: Display of surfaces from volume data. IEEE Computer Graphics and Applications, 8(5):29-37, 1988. 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