Naslov
Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?

Autori
Klobučar, Göran Igor Vinko ; Štambuk, Anamaria ; Šrut, Maja ; Husnjak, Ivana ; Merkaš, Martina ; Traven, Luka ; Cvetković, Želimira

Izvornik
Environmental pollution (0269-7491) 159 (2011), 4; 841-849

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Comet assay; DNA damage; NRRT assay; coelomocytes; Aporrectodea caliginosa; Eisenia fetida

Sažetak
There is a growing interest for the application of biomakers to field collected earthworms. Therefore we have evaluated the usability of native populations of endogeic, widely distributed earthworm Aporrectodea caliginosa in the assessment of soil genotoxicity using the Comet assay. Validation of the Comet assay on earthworm coelomocytes has been established using commercially available Eisenia fetida exposed to copper, cadmium, and pentachlorophenol, along with A. caliginosa exposed to copper in a filter paper contact test. Neutral red retention time (NRRT) assay was conducted on copper exposed and field collected earthworms. Significant DNA and lysosomal damage was measured using Comet and NRRT assays in native populations of A. caliginosa sampled from the polluted soils in the urban area in comparison to the earthworms from the reference site. The results of this study confirm employment of A. caliginosa as a suitable species for the in situ soil toxicity and genotoxicity field surveys.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA