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Pregled bibliografske jedinice broj: 493718

Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins

Brgles, Marija; Clifton, James; Walsh, Robert; Huang, Feilei; Ručević, Marijana; Lulu, Cao; Hixson, Douglas; Müller, Egberg; Josić, Djuro
Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins // Journal of chromatography. A, 1218 (2011), 17; 2389-2395 doi:10.1016/j.chroma.2010.11.059 (međunarodna recenzija, članak, znanstveni)

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Naslov
Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins

Autori
Brgles, Marija ; Clifton, James ; Walsh, Robert ; Huang, Feilei ; Ručević, Marijana ; Lulu, Cao ; Hixson, Douglas ; Müller, Egberg ; Josić, Djuro

Izvornik
Journal of chromatography. A (0021-9673) 1218 (2011), 17; 2389-2395

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
monoliths; plasma; chromatography

Sažetak
Human serum albumin (HSA) and immunoglobulin G (IgG) represent over 75% of all proteins present in human plasma. These two proteins frequently interfere with detection, determination and purification of low abundance proteins that can be potential biomarkers and biomarker candidates for various diseases. Some low abundance plasma proteins such as clotting factors and inhibitors are also important therapeutic agents. In this paper, the characterization of ion-exchange monolithic supports under overloading conditions was performed by use of sample displacement chromatography (SDC). If these supports were used for separation of human plasma, the composition of bound and eluted proteins in both anion and cation-exchange mode is dependent on column loading. Under overloading conditions, the weakly bound proteins such as HSA in anion exchange and IgG in cation-exchange mode seem to be are displaced by stronger binding proteins, and this phenomenon was not dependent on column size. Consequently, small monolithic columns with a column volume of 100 and 200 μL are ideal supports for high throughput screening in order to develop new methods for separation of complex mixtures, and for sample preparation in proteomic technology.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

Napomena
U časopisu su objavljeni odabrani radovi prezentirani na skupu The 4th Summer School of Monolith Technology for Biochromatography, Bioconversion and Phase State Synthesis, održanom od 30.05.-02.06.2010., Portorož, Slovenija. Edited by R. Giese



POVEZANOST RADA

Ustanove:
Imunološki zavod d.d.

Profili:

Avatar Url Marijana Ručević (autor)

Avatar Url Marija Brgles (autor)

Avatar Url Đuro Josić (autor)

Poveznice na cjeloviti tekst rada:

doi ac.els-cdn.com www.sciencedirect.com

Citiraj ovu publikaciju:

Brgles, Marija; Clifton, James; Walsh, Robert; Huang, Feilei; Ručević, Marijana; Lulu, Cao; Hixson, Douglas; Müller, Egberg; Josić, Djuro
Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins // Journal of chromatography. A, 1218 (2011), 17; 2389-2395 doi:10.1016/j.chroma.2010.11.059 (međunarodna recenzija, članak, znanstveni)
Brgles, M., Clifton, J., Walsh, R., Huang, F., Ručević, M., Lulu, C., Hixson, D., Müller, E. & Josić, D. (2011) Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins. Journal of chromatography. A, 1218 (17), 2389-2395 doi:10.1016/j.chroma.2010.11.059.
@article{article, author = {Brgles, Marija and Clifton, James and Walsh, Robert and Huang, Feilei and Ru\v{c}evi\'{c}, Marijana and Lulu, Cao and Hixson, Douglas and M\"{u}ller, Egberg and Josi\'{c}, Djuro}, year = {2011}, pages = {2389-2395}, DOI = {10.1016/j.chroma.2010.11.059}, keywords = {monoliths, plasma, chromatography}, journal = {Journal of chromatography. A}, doi = {10.1016/j.chroma.2010.11.059}, volume = {1218}, number = {17}, issn = {0021-9673}, title = {Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins}, keyword = {monoliths, plasma, chromatography} }
@article{article, author = {Brgles, Marija and Clifton, James and Walsh, Robert and Huang, Feilei and Ru\v{c}evi\'{c}, Marijana and Lulu, Cao and Hixson, Douglas and M\"{u}ller, Egberg and Josi\'{c}, Djuro}, year = {2011}, pages = {2389-2395}, DOI = {10.1016/j.chroma.2010.11.059}, keywords = {monoliths, plasma, chromatography}, journal = {Journal of chromatography. A}, doi = {10.1016/j.chroma.2010.11.059}, volume = {1218}, number = {17}, issn = {0021-9673}, title = {Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins}, keyword = {monoliths, plasma, chromatography} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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