Naslov
Effect of high hydrostatic pressure on mortality and allergenicity of Anisakis simplex L3 and on muscle properties of infested hake

Autori
Vidaček, Sanja ; de las Heras, Cristina ; Solas, Maria Teresa ; Rodriguez Mahillo, Ana ; Tejada, Margarita

Izvornik
Journal of the science of food and agriculture (0022-5142) 89 (2009), 13; 2228-2235

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Anisakis simplex; allergen; fish muscle; high pressure; hake; SEM; IHC

Sažetak
High pressure (HP) ranging from 100 to 350 MPa (1–15 min) was applied to Anisakis simplex larvae and parasitised hake (Merluccius merluccius) muscle. The aim of the study was to kill the larvae to prevent human anisakidosis, to evaluate the effect on A. simplex allergens and to minimally alter fish muscle quality. The larvae were killed at pressures ≥200 MPa and times ≥1 min, producing alterations in the larva body and ruptures in the cuticle when observed by scanning electron microscopy. Nevertheless, Ani s 4 and A. simplex crude antigens were recognised by immunoblotting and immunohistochemistry at all HPs assayed. Small changes in colour and texture were observed in fishmuscle under all pressure/time conditions. Major changes were observed visually at 300 MPa, where the muscle appeared as slightly cooked. Apparent viscosity of muscle homogenates decreased significantly at longer times or higher applied pressure. No changes were detected at 200 MPa in the electrophoretic pattern of proteins treated with or without β-mercaptoethanol, suggesting that disulfide bonds were not formed. Application of HP at 200 MPa for up to 5min would kill A. simplex larvae, avoiding infestation of the consumer and causing small changes in the hakemuscle perceived sensorially. However, HP-treated A. simplex-parasitised fishwould still be a potential hazard for consumers allergic to the larvae.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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