Naslov
TP53 gene polymorphisms and breast cancer in Croatian women: a pilot study

Autori
Bišof, Vesna ; Peričić Salihović, Marijana ; Smolej Narančić, Nina ; Škarić-Jurić, Tatjana ; Jakić-Razumović, Jasminka ; Janićijević, Branka ; Turek, Stjepan ; Rudan, Pavao

Izvornik
European journal of gynaecological oncology (0392-2936) 31 (2010), 5; 539-544

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
breast cancer; polymorphism; TP53 gene; Croatia

Sažetak
A case-control retrospective association study was conducted to investigate a possible association of the TP53 polymorphisms, Arg72Pro and PIN3 (+16bp), with sporadic breast cancer in Croatian women. Ninety-five women with breast cancer and 108 age-matched healthy women were analyzed. Arg72Pro polymorphism was detected by TaqMan essay. For designation of PIN3 (+16bp) polymorphism DNA amplification was performed by the polymerase chain reaction (PCR) while the PCR products were detected by capillary electrophoresis. Homozygous genotype of minor allele of the PIN3 (+16bp) polymorphism was associated with sporadic breast cancer (OR=2.15, 95% confidence interval [CI] 1.80-2.56, p=0.006). For Arg72 polymorphism, the odds ratio for breast cancer of ArgPro versus reference genotype ArgArg was 0.55 (95% CI 0.30-1.02, p=0.039) suggesting the protective effect. Although different haplotypes did not influence the susceptibility to the disease, the joint occurrence of genotype combination ProPro/A2A2 frequent in cases, was associated with sporadic breast cancer (OR=2.20, 95% CI 1.89-2.56, p=0.021). The study provides evidence of the association of the TP53 gene Arg72Pro and PIN3 (+16bp) polymorphisms with sporadic breast cancer in the Croatian population.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Etnologija i antropologija

POVEZANOST RADA