Naslov
Deoxyribonucleases (DNases) in the cortex and endosome from marine sponge Tethya aurantium

Autori
Fafanđel, Maja ; Ravlić, Sanda ; Smodlaka, Mirta ; Bihari, Nevenka

Izvornik
Russian Journal of Marine Biology (1063-0740) 36 (2010), 5; 383-389

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
deoxyribonucleases ; cortex ; endosome ; marine sponge ; Tethya aurantium ; acid DNase ; neutral DNase

Sažetak
The presence and activity of deoxyribonucleases in the cortex and endosome sections from a sponge, the sea orange Tethya aurantium, were investigated. The maximal enzyme activity in sponge homogenate was detected at pH 4.27, pH 7.0 and pH 8.5–8.75. Among different specimens, several distinct patterns of neutral DNase isozymes were observed in the cortex section. In each investigated specimen the highest neutral DNase activity belonged to high molecular weight proteins (up to75 kDa). The acid DNases showed a low level of enzyme activity. In the endosome section the acid DNase activity was up to ten times higher than in the cortex and the presence of DNase II-like protein was detected. Neutral DNase, which expressed the highest enzyme activity in all the investigated specimens, has a molecular weight of 20 kDa and belongs to the DNase I-like family. The results indicate that the activity of neutral and acid DNases is related to sponge sections and their biological functions. The cortex, as the sponge section that communicates with the environment, expresses high interindividual variability and heterogeneity of neutral DNases, while the endosome section, where the intracellular digestion is localized, is a site of high acid DNase activity.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

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