Pregled bibliografske jedinice broj: 489816
Poly(ADP)ribose polymerase in the biology of glioblastoma cells
Poly(ADP)ribose polymerase in the biology of glioblastoma cells // Book of Abstracts of "HDIR-1: From Bench to Clinic" - First Meeting of the Croatian Association for Cancer Research with International Participation / Sabol, Maja ; Levanat, Sonja (ur.).
Zagreb: Hrvatsko društvo za istraživanje raka (HDIR), 2010. str. 77-77 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 489816 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Poly(ADP)ribose polymerase in the biology of glioblastoma cells
Autori
Madunić, Josip ; Antica, Mariastefania ; Matulić, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts of "HDIR-1: From Bench to Clinic" - First Meeting of the Croatian Association for Cancer Research with International Participation
/ Sabol, Maja ; Levanat, Sonja - Zagreb : Hrvatsko društvo za istraživanje raka (HDIR), 2010, 77-77
Skup
"HDIR-1: From Bench to Clinic" - First Meeting of the Croatian Association for Cancer Research with International Participation
Mjesto i datum
Zagreb, Hrvatska, 11.11.2010
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
poly(ADP)ribose polymerase
Sažetak
Poly(ADP)polymerase-1 (PARP-1) is a nuclear protein involved in the posttranslational process of poly(ADPribos)ylation, synthesis of ADP-ribose polymer on target proteins. It has an important role in maintaining genome integrity. Its enzymatic activity is highly induced by DNA strand breaks, when it recruits damage repair proteins to the DNA. PARP-1 has also a role in the regulation of transcription and chromatin structure, as well as in DNA methylation. In our study we investigated the role of PARP-1 in the biology of the A1235 human glioblastoma cell line. Cells were treated with sublethal doses of alkylating agent N-methyl-N'-nitro- N-nitrosoguanidine (MNNG), either alone or in combination with PARP-1 inhibitor. We analyzed cell proliferation, appearance of senescent cells, changes in cell morphology, cell cycle and effects on the urokinase plasminogen activator induction. Although PARP-1 inhibitors slightly decreased cell proliferation when added with MNNG in comparison with the alkylating agent alone, both treatments similarly affected cell cycle (accumulation of cells in S and G2 cell cycle phase) and also induced senescence. On the other side, activity of the extracellular protease, urokinase plasminogen activator, known to be induced in A1235 cells by alkylating agents, was modulated by the PARP-1 inhibitor. The mechanisms of PARP-1 regulation in the process of transcription will be discussed.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
098-0982913-2332 - Molekularne interakcije u diferencijaciji limfocita (Antica, Mariastefania, MZOS ) ( CroRIS)
119-0000000-3172 - Poli(ADPribozilacija, starenje i plazminogenska aktivacija u tumorskim stanicama (Matulić, Maja, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb