Naslov
Impact of molecular environment on the lipid oxidation induced by ferrous ion

Autori
Tartaro, Ivana ; Mihaljević, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SFRR-E Free Radical Summer School "Lipid peroxidation and free radical signaling: role in pathophysiology" / Ozer, Nesrin K. - , 2008

Skup
Free Radical Summer School "Lipid peroxidation and free radical signaling: role in pathophysiology"

Mjesto i datum
Spétses, Grčka, 30.08.2008. - 05.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
lipid peroxidation; mixed micelles; ferrous ion

Sažetak
Lipid hydroperoxides (LOOH) present even in traces, easily degrade under effects of different initiators including transition metals. In previous lipid peroxidation investigations, the ferrous addition to lipid systems caused the simultaneous production of hydroxyl and alkoxyl radicals, leading to the propagation of lipid oxidation process. Consequently, the individual steps in lipid peroxidation could not be distinguished. This was the reason for rather difficult interpretations and disagreements between the molecular mechanisms proposed for the different phases of peroxidation. We have established the simple model system which allows the selective study of the propagation of lipid peroxidation. Mixed micelles formed from nonionic surfactant (Tween 20) and polyunsaturated fatty acids (PUFA) have been used as a model system in which autoxidation of ferrous ions and thereby reactive oxygen species generation does not occur. Various subsystems were analyzed in order to better understand the involved processes. Decreasing pH makes iron(II) and iron(III) ions more available for oxidative processes. With exception of the normal tissue of the gastric system having ordinary high acidity, in comparison with normal tissue, tumors have relatively low pH levels. Thus, it is important to consider the mechanism of LH oxidation under conditions other than normal physiological ones. Under optimum experimental conditions, oxidative susceptibility of different PUFA under influence of ferrous ion, with a special attention given to the oxidation rate as affected by the ferrous concentration, will be determined. It was found that the oxidative stability of PUFA decreases with increasing number of double bonds or number of bis-alylic position. The susceptibility of PUFA to peroxidation in aqueous micellar solution could be associated with their individual conformations. Dependence of linoleic acid peroxidation on ferrous concentrations revealed that chosen model allow study of either the propagation or the termination phase of lipid peroxidation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA