Pregled bibliografske jedinice broj: 488716
Intracellular uptake and intraspheroidal distribution of hypericin and hydrophilic analogues using E-cadherin transfected T-24 human bladder cancer cells
Intracellular uptake and intraspheroidal distribution of hypericin and hydrophilic analogues using E-cadherin transfected T-24 human bladder cancer cells // Proceedings of SPIE
San Jose (CA), Sjedinjene Američke Države, 2008. (predavanje, nije recenziran, cjeloviti rad (in extenso), znanstveni)
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Naslov
Intracellular uptake and intraspheroidal distribution of hypericin and hydrophilic analogues using E-cadherin transfected T-24 human bladder cancer cells
Autori
Crnolatac, Ivo ; Huygens, Ann ; de Witte, Peter
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Proceedings of SPIE
/ - , 2008
Skup
SPIE International symposium on Biomedical Optics
Mjesto i datum
San Jose (CA), Sjedinjene Američke Države, 19.01.2008. - 24.01.2008
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Nije recenziran
Ključne riječi
hypericin; photodynamic diagnosis; photodynamic therapy; transitional cell carcinoma; bladder cancer; spheroids; intracellular uptake; paracellular transport
Sažetak
Hypericin (HYP) is used after instillation as a diagnostic tool for the fluorescence detection of CIS in the human bladder. In this study the in vitro cellular accumulation and intraspheroidal distribution of HYP and three analogues (OH1, OH2, OH3) with gradually increasing hydrophilicity were studied. E-cadherin negative (T24-C1-) and E-cadherin positive (T24-H3++) human bladder cancer cells were used. We report that in the presence of FBS all compounds were taken up by the monolayer cells to the same limited extent, whereas the overall intracellular accumulation was substantially higher when the incubation of the different dyes took place using cell medium not supplemented with FBS. The results of this study therefore confirm the competition between cellular uptake of HYP and analogues and binding to FBS constituents. Investigating the permeation of the compounds in spheroids, it was found that all HYP analogues diffused dramatically better through the three-dimensional cell layers than HYP itself. This enhanced ability of hydrophilic HYP analogues to permeate through the cell layers in the presence of FBS can be explained in terms of a preferred binding to HDL as compared to LDL. The results further show that all compounds, including LDL-binding HYP, substantially permeated better in T24-C1- spheroids than in T24-H3++ spheroids. The data therefore support the hypothesis that a lowered cellular cohesion is the key to understand the selective uptake of hypericin and its analogues in malignant urothelial cells.
Izvorni jezik
Engleski
