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Pregled bibliografske jedinice broj: 488709

Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin

Huygens, Ann; Crnolatac, Ivo; Develter, Jan; Van Cleynenbreugel, Ben; Van der Kwast, Theo; de Witte, Peter
Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin // The Journal of urology, 179 (2008), 5; 2014-2019 doi:10.1016/j.juro.2007.12.038 (međunarodna recenzija, članak, znanstveni)

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Naslov
Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin

Autori
Huygens, Ann ; Crnolatac, Ivo ; Develter, Jan ; Van Cleynenbreugel, Ben ; Van der Kwast, Theo ; de Witte, Peter

Izvornik
The Journal of urology (0022-5347) 179 (2008), 5; 2014-2019

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bladder; carcinoma; transitional cell; cadherins; hypericin; administration; intravesical

Sažetak
To obtain unambiguous evidence for the putative role of E-cadherin in the selective accumulation of hypericin after intravesical instillation in humans we investigated the accumulation of hypericin in spheroids from 3 clones of the human bladder carcinoma cell line T-24 that express different levels of E-cadherin, as determined by immunohistochemistry and reverse transcriptase-polymerase chain reaction. Clones of T-24 cells transfected with the E-cadherin gene were analyzed for E-cadherin expression and 3 cell lines with different expression levels were selected. Spheroids of these cell lines were incubated with 10 μM hypericin in cell culture medium supplemented or not with fetal calf serum for 2 hours. After the incubation period centrally cut sections were examined by fluorescence microscopy. An imaging software system was used to measure average fluorescence in concentric layers from rim to center. Results Data showed that in the presence of serum the accumulation of hypericin in spheroids was inversely associated with the level of E-cadherin expressed by the T-24 transfectants used, whereas in the absence of serum differential accumulation of the compound was completely abolished. Spheroids composed of cancer cell lines expressing variable levels of E-cadherin represent an excellent model in which to study the role of intercellular adhesion in bladder cancer. The outcome of this study strongly suggests that E-cadherin is the key mediator in the selective accumulation of hypericin in superficial bladder cancer after intravesical instillation in humans.

Izvorni jezik
Engleski



POVEZANOST RADA

Profili:

Avatar Url Ivo Crnolatac (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Huygens, Ann; Crnolatac, Ivo; Develter, Jan; Van Cleynenbreugel, Ben; Van der Kwast, Theo; de Witte, Peter
Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin // The Journal of urology, 179 (2008), 5; 2014-2019 doi:10.1016/j.juro.2007.12.038 (međunarodna recenzija, članak, znanstveni)
Huygens, A., Crnolatac, I., Develter, J., Van Cleynenbreugel, B., Van der Kwast, T. & de Witte, P. (2008) Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin. The Journal of urology, 179 (5), 2014-2019 doi:10.1016/j.juro.2007.12.038.
@article{article, author = {Huygens, Ann and Crnolatac, Ivo and Develter, Jan and Van Cleynenbreugel, Ben and Van der Kwast, Theo and de Witte, Peter}, year = {2008}, pages = {2014-2019}, DOI = {10.1016/j.juro.2007.12.038}, keywords = {bladder, carcinoma, transitional cell, cadherins, hypericin, administration, intravesical}, journal = {The Journal of urology}, doi = {10.1016/j.juro.2007.12.038}, volume = {179}, number = {5}, issn = {0022-5347}, title = {Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin}, keyword = {bladder, carcinoma, transitional cell, cadherins, hypericin, administration, intravesical} }
@article{article, author = {Huygens, Ann and Crnolatac, Ivo and Develter, Jan and Van Cleynenbreugel, Ben and Van der Kwast, Theo and de Witte, Peter}, year = {2008}, pages = {2014-2019}, DOI = {10.1016/j.juro.2007.12.038}, keywords = {bladder, carcinoma, transitional cell, cadherins, hypericin, administration, intravesical}, journal = {The Journal of urology}, doi = {10.1016/j.juro.2007.12.038}, volume = {179}, number = {5}, issn = {0022-5347}, title = {Differential Accumulation of Hypericin in Spheroids Composed of T-24 Transitional Cell Carcinoma Cells Expressing Different Levels of E-Cadherin}, keyword = {bladder, carcinoma, transitional cell, cadherins, hypericin, administration, intravesical} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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