Naslov
Histone modification levels are predictive of gene expression

Autori
Karlić, Rosa ; Chung, Ho-Ryun ; Lasserre, Julia ; Vlahoviček, Kristian ; Vingron, Martin.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
IRI chromatin days : Chromatin Remodeling

Mjesto i datum
Lille, Francuska, 07.10.2010. - 08.10.2010

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
histone modifications; regression analysis; transcription

Sažetak
Histones are frequently decorated with covalent modifications, which are thought to be involved in various chromatin-dependent processes, including transcription. Qualitative correlations between the presence or absence of certain histone modifications at the promoter and gene expression have been reported before.We used the levels of histone modifications at the promoter to predict the expression level of corresponding genes. Our model was able to faithfully predict gene expression suggesting that the levels of histone modifications are quantitatively related to the expression level. Furthermore, a model trained on one cell type was able to predict gene expression of another cell type using the levels of histone modifications measured in this other cell type. This result indicates that the uncovered relationships between histone modifications and gene expression are general. A more detailed analysis revealed that only a small number of important histone modifications is necessary to accurately predict gene expression. These important modifications are different for genes driven by high CpG content promoters and low CpG content promoters. Taken together, we propose that histone modifications are very close to RNA Polymerase II in the regulatory network controlling its activity, but our analysis does not allow for deciding whether histone modifications are cause or consequence of transcription. Our findings could greatly reduce the experimental effort to study the relationship between histone modifications and transcription, and could potentially be viewed as the starting point for establishing a "histone code of transcription".

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA