Naslov
Ikaros and Notch transcription factors in lymphocyte differentiation

Autori
Paradžik, Mladen ; Matulić, Maja ; Batinić, Drago ; Labar, Boris ; Antica, Mariastefania

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDIR / - , 2010

Skup
Croatian Association for Cancer Research First meeting with international participation

Mjesto i datum
Zagreb, Hrvatska, 11.11.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Ikaros; Notch; lymphocytes; differentiation

Sažetak
Leukemic cells arise from cells stopped at various developmental stages during differentiation. We study the mechanisms of leukemia development by analyzing genes involved in hematopoietic differentiation. Gene targeting studies show that major players in the commitment of hematopoietic progenitors are Ikaros and Notch transcription factors and inappropriate expression of these genes might be involved in leukemogenesis. Accordingly, we analyzed the differential expression of Ikaros transcription factors and their splicing variants in the most frequent leukemia types, CLL, ALL and AML and in hematological cell lines. By means of qRT-PCR we show that mRNA expression of Ikaros, Aiolos and Helios varies in our samples. We provide evidence that splicing variants of these transcription factors occur but they are not restricted to leukemic cells only. Further, we show here that the Notch ligand DL1 has an important role in the induced differentiation of leukemic cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA